Quantitative microCT imaging of a whole equine placenta and its blood vessel network
Quantitative microCT imaging of a whole equine placenta and its blood vessel network
Placental structure is linked to function across morphological scales. In the placenta, changes to gross anatomy, such as surface area, volume, or blood vessel arrangement, are associated with suboptimal physiological outcomes. However, quantifying each of these metrics requires different laborious semi-quantitative methods. Here, we demonstrate how, with minimal sample preparation, whole-organ computed microtomography (microCT) can be used to calculate gross morphometry of the equine placenta and a range of additional metrics, including branching morphometry of placental vasculature, non-destructively from a single dataset. Our approach can be applied to quantify the gross structure of any large mammalian placenta.
Equine, Placenta, microCT
216-219
Laundon, Davis
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Proudley, Ella
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Basford, Philip J.
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Katsamenis, Orestis L.
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Chatelet, David S.
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Cleal, Jane K
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Gostling, Neil J.
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Chavatte-Palmer, Pascale
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Lewis, Rohan M.
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2 August 2024
Laundon, Davis
b2ba5687-d949-47dd-b84f-fb3c0c032550
Proudley, Ella
37a16fbc-e5e9-40f9-805a-df6d514b5aa2
Basford, Philip J.
d65c2fe2-adab-4d34-a346-af9a7164e034
Katsamenis, Orestis L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Chatelet, David S.
6371fd7a-e274-4738-9ccb-3dd4dab32928
Cleal, Jane K
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Gostling, Neil J.
4840aa40-cb6c-4112-a0b9-694a869523fc
Chavatte-Palmer, Pascale
3b1ff263-828e-4f55-ab66-13168e3ed45f
Lewis, Rohan M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Laundon, Davis, Proudley, Ella, Basford, Philip J., Katsamenis, Orestis L., Chatelet, David S., Cleal, Jane K, Gostling, Neil J., Chavatte-Palmer, Pascale and Lewis, Rohan M.
(2024)
Quantitative microCT imaging of a whole equine placenta and its blood vessel network.
Placenta, 154, .
(doi:10.1016/j.placenta.2024.07.313).
Abstract
Placental structure is linked to function across morphological scales. In the placenta, changes to gross anatomy, such as surface area, volume, or blood vessel arrangement, are associated with suboptimal physiological outcomes. However, quantifying each of these metrics requires different laborious semi-quantitative methods. Here, we demonstrate how, with minimal sample preparation, whole-organ computed microtomography (microCT) can be used to calculate gross morphometry of the equine placenta and a range of additional metrics, including branching morphometry of placental vasculature, non-destructively from a single dataset. Our approach can be applied to quantify the gross structure of any large mammalian placenta.
Text
1-s2.0-S0143400424006088-main
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Accepted/In Press date: 31 July 2024
e-pub ahead of print date: 31 July 2024
Published date: 2 August 2024
Keywords:
Equine, Placenta, microCT
Identifiers
Local EPrints ID: 493371
URI: http://eprints.soton.ac.uk/id/eprint/493371
ISSN: 0143-4004
PURE UUID: f0be7733-b80b-4e17-ac63-e54143dc92b7
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Date deposited: 30 Aug 2024 16:56
Last modified: 31 Aug 2024 02:06
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Contributors
Author:
Davis Laundon
Author:
Ella Proudley
Author:
Philip J. Basford
Author:
David S. Chatelet
Author:
Pascale Chavatte-Palmer
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