Bioactive coatings on 3D printed scaffolds for bone regeneration: use of Laponite® to deliver BMP-2 in an ovine femoral condyle defect model: Use of Laponite® to deliver BMP-2 in an ovine femoral condyle defect model
Bioactive coatings on 3D printed scaffolds for bone regeneration: use of Laponite® to deliver BMP-2 in an ovine femoral condyle defect model: Use of Laponite® to deliver BMP-2 in an ovine femoral condyle defect model
Biomaterial-based approaches for bone regeneration seek to explore alternative strategies to repair non-healing fractures and critical-sized bone defects. Fracture non-union occurs due to a number of factors resulting in the formation of bone defects. Rigorous evaluation of the biomaterials in relevant models and assessment of their potential to translate towards clinical use is vital. Large animal experimentation can be used to model fracture non-union while scaling-up materials for clinical use. Growth factors modulate cell phenotype, behaviour and initiate signalling pathways leading to changes in matrix deposition and tissue formation. Bone morphogenetic protein-2 (BMP-2) is a potent osteogenic growth factor, with a rapid clearance time in vivo necessitating clinical use at a high dose, with potential deleterious side-effects. The current studies have examined the potential for Laponite® nanoclay coated poly(caprolactone) trimethacrylate (PCL-TMA900) scaffolds to bind BMP-2 for enhanced osteoinduction in a large animal critical-sized bone defect. An ovine femoral condyle defect model confirmed PCL-TMA900 scaffolds coated with Laponite®/BMP-2 produced significant bone formation compared to the uncoated PCL-TMA 900 scaffold in vivo, assessed by micro-computed tomography (μCT) and histology. This indicated the ability of Laponite® to deliver the bioactive BMP-2 on the PCL-TMA900 scaffold. Bone formed around the Laponite®/BMP-2 coated PCL-TMA900 scaffold, with no erroneous bone formation observed away from the scaffold material confirming localisation of BMP-2 delivery. The current studies demonstrate the ability of a nanoclay to localise and deliver bioactive BMP-2 within a tailored octet-truss scaffold for efficacious bone defect repair in a large animal model with significant implications for translation to the clinic.
BMP-2, Bone tissue engineering, Femoral defect model, Laponite®, Poly(caprolactone), Sheep
Marshall, Karen M.
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McLaren, Jane S.
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Wojciechowski, Jonathan P.
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Callens, Sebastien J.P.
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Echalier, Cécile
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Kanczler, Janos M.
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Rose, Felicity R.A.J.
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Stevens, Molly M.
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Dawson, Jonathan I.
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Oreffo, Richard O.C.
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30 July 2024
Marshall, Karen M.
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McLaren, Jane S.
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Wojciechowski, Jonathan P.
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Callens, Sebastien J.P.
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Echalier, Cécile
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Kanczler, Janos M.
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Rose, Felicity R.A.J.
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Stevens, Molly M.
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Dawson, Jonathan I.
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Oreffo, Richard O.C.
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Marshall, Karen M., McLaren, Jane S., Wojciechowski, Jonathan P., Callens, Sebastien J.P., Echalier, Cécile, Kanczler, Janos M., Rose, Felicity R.A.J., Stevens, Molly M., Dawson, Jonathan I. and Oreffo, Richard O.C.
(2024)
Bioactive coatings on 3D printed scaffolds for bone regeneration: use of Laponite® to deliver BMP-2 in an ovine femoral condyle defect model: Use of Laponite® to deliver BMP-2 in an ovine femoral condyle defect model.
Biomaterials Advances, 164, [213959].
(doi:10.1016/j.bioadv.2024.213959).
Abstract
Biomaterial-based approaches for bone regeneration seek to explore alternative strategies to repair non-healing fractures and critical-sized bone defects. Fracture non-union occurs due to a number of factors resulting in the formation of bone defects. Rigorous evaluation of the biomaterials in relevant models and assessment of their potential to translate towards clinical use is vital. Large animal experimentation can be used to model fracture non-union while scaling-up materials for clinical use. Growth factors modulate cell phenotype, behaviour and initiate signalling pathways leading to changes in matrix deposition and tissue formation. Bone morphogenetic protein-2 (BMP-2) is a potent osteogenic growth factor, with a rapid clearance time in vivo necessitating clinical use at a high dose, with potential deleterious side-effects. The current studies have examined the potential for Laponite® nanoclay coated poly(caprolactone) trimethacrylate (PCL-TMA900) scaffolds to bind BMP-2 for enhanced osteoinduction in a large animal critical-sized bone defect. An ovine femoral condyle defect model confirmed PCL-TMA900 scaffolds coated with Laponite®/BMP-2 produced significant bone formation compared to the uncoated PCL-TMA 900 scaffold in vivo, assessed by micro-computed tomography (μCT) and histology. This indicated the ability of Laponite® to deliver the bioactive BMP-2 on the PCL-TMA900 scaffold. Bone formed around the Laponite®/BMP-2 coated PCL-TMA900 scaffold, with no erroneous bone formation observed away from the scaffold material confirming localisation of BMP-2 delivery. The current studies demonstrate the ability of a nanoclay to localise and deliver bioactive BMP-2 within a tailored octet-truss scaffold for efficacious bone defect repair in a large animal model with significant implications for translation to the clinic.
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Accepted/In Press date: 14 July 2024
e-pub ahead of print date: 18 July 2024
Published date: 30 July 2024
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© 2024 The Authors
Keywords:
BMP-2, Bone tissue engineering, Femoral defect model, Laponite®, Poly(caprolactone), Sheep
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Local EPrints ID: 493441
URI: http://eprints.soton.ac.uk/id/eprint/493441
ISSN: 2772-9508
PURE UUID: 6893d40a-169d-4801-ab95-7ebbc3d3bf7e
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Date deposited: 03 Sep 2024 16:36
Last modified: 16 Oct 2024 02:01
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Contributors
Author:
Karen M. Marshall
Author:
Jane S. McLaren
Author:
Jonathan P. Wojciechowski
Author:
Sebastien J.P. Callens
Author:
Cécile Echalier
Author:
Janos M. Kanczler
Author:
Felicity R.A.J. Rose
Author:
Molly M. Stevens
Author:
Jonathan I. Dawson
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