READ ME File For: Raw Datasets for PhD thesis entitled Coastal-Estuarine Microbiology Explored: Community Dynamics, Biogeochemical Processes and Cell Specific Targeting of Nitrogen Cyclers

Dataset DOI:  https://doi.org/10.5258/SOTON/D2923

ReadMe Author: Spencer Long, University of Southampton ORCID ID

This dataset supports the thesis entitled 

Coastal-Estuarine Microbiology Explored: Community Dynamics, Biogeochemical Processes and Cell Specific Targeting of Nitrogen Cyclers

AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2024

DESCRIPTION OF THE DATA

These datasets contain raw data for the PhD thesis entitled "Coastal-Estuarine Microbiology Explored: Community Dynamics, Biogeochemical Processes and Cell Specific Targeting of Nitrogen Cyclers", authored by Spencer Long.

Datasets are as follows:

- tamar_nutrients_master.csv contains raw nutrient data from the Tamar estuary, UK, for dates sampled in September 2019, March 2020, September 2021 and May 2022. Obtained via various methods of nutrient analysis.

-  L4_nutrients_master.csv contains raw nutrient data from Western Channel Observatory station L4, for dates sampled in September 2019, March 2020 and May 2022. Obtained via various methods of nutrient analysis.

- nitrifier_CARD_stats.csv contains catalysed deposition fluorescence in situ hybridisation (CARD-FISH) obtained raw data for ammonia oxidising microbial communities within thew Tamar estuary. Data relates to samples taken in September 2019, March 2020, September 2021 and May 2022. Obtained via epilfuorescence microscopy counting. 

- nitrification_rate.csv contains calculated ammonia oxidation rate raw data for the Tamar estaury, relating to samples taken in September 2021 and May 2022. Obtained via nutrient analysis via an auto analyser.

- plate_reader_master.csv contains raw data obtained from all plate reader experiments relating to the chapter "Assessing the Applicability of DAF-FM Diacetate for the Specific Detection of Nitric Oxide Cycling Microorganisms". Obtained from plate reader.

- fluor_spreadsheet_final.csv contains raw data obtained from all epifluorescence microscopy experiments relating to the chapter "Assessing the Applicability of DAF-FM Diacetate for the Specific Detection of Nitric Oxide Cycling Microorganisms". Obtained via epifluorescence microscopy


This dataset contains:

tamar_nutrients_master.csv
L4_nutrients_master.csv
nitrifier_CARD_stats.csv 
nitrification_rate.csv 
plate_reader_master.csv
fluor_spreadsheet_final.csv 

Date of data collection: 16th September 2019 - 12 December 2023

Information about geographic location of data collection: 

Licence:
NA

Related projects/Funders:
Data collected as part of the SPITFIRE Doctoral Training Programme, funded by NERC grant NE/L002531/1 



Date that the file was created: January 2024