Using laponite to deliver BMP-2 for bone tissue engineering – in vitro, chorioallantoic membrane assay and murine subcutaneous model validation
Using laponite to deliver BMP-2 for bone tissue engineering – in vitro, chorioallantoic membrane assay and murine subcutaneous model validation
Fracture non-union occurs due to various factors, leading to the development of potentially substantial bone defects. While autograft and allograft are the current gold standards for non-union fractures, challenges related to availability and immune rejection highlight the need for improved treatments. A strategy in bone tissue engineering is to harness growth factors to induce an effect on cells to change their phenotype, behavior and initiate signaling pathways which lead to increased matrix deposition and tissue formation. Bone morphogenetic protein-2 (BMP-2) is a potent osteogenic growth factor however, given its rapid clearance time in vivo, there is a specific therapeutic window for efficacy while avoiding potential deleterious side-effects. It is demonstrated that a Laponite nanoclay coating on a 3D printable and bioresorbable poly(caprolactone) trimethacrylate-based resin enables binding of BMP-2, decreases the rate of release, enabling reduced concentrations to be used while enhancing osteoinduction in both in vitro and in vivo models.
biomaterial, bone tissue engineering, CAM assay, Laponite, subcutaneous implantation model
Marshall, Karen M.
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Wojciechowski, Jonathan P.
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Echalier, Cécile
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Callens, Sebastien J.P.
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Yang, Tao
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Øvrebø, Øystein
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Zhou, Kun
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Jayawarna, Vineetha
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Kanczler, Janos M.
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Stevens, Molly M.
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Dawson, Jonathan I.
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Oreffo, Richard O.C.
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29 July 2024
Marshall, Karen M.
955e07ec-09e2-4464-aca7-65351afe19e3
Wojciechowski, Jonathan P.
7c275478-97aa-45f0-9e92-11443e4cf030
Echalier, Cécile
04700d94-231d-4a9e-a40d-5f5f6b42a42b
Callens, Sebastien J.P.
f703e6d7-dbfc-4174-a98c-30e539862ae5
Yang, Tao
08a09622-7c46-495a-ba4e-c56b7e40bbb5
Øvrebø, Øystein
9dab3111-d892-47f7-91b8-58380a34951c
Zhou, Kun
2bb82231-b6ad-4b96-9b3a-ff3c305436b1
Jayawarna, Vineetha
aa5cc11a-81a3-486f-b375-6bcf0be460ec
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Stevens, Molly M.
2af17549-764e-4c18-a316-f7dc790398e0
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Marshall, Karen M., Wojciechowski, Jonathan P., Echalier, Cécile, Callens, Sebastien J.P., Yang, Tao, Øvrebø, Øystein, Zhou, Kun, Jayawarna, Vineetha, Kanczler, Janos M., Stevens, Molly M., Dawson, Jonathan I. and Oreffo, Richard O.C.
(2024)
Using laponite to deliver BMP-2 for bone tissue engineering – in vitro, chorioallantoic membrane assay and murine subcutaneous model validation.
Advanced Materials Interfaces.
(doi:10.1002/admi.202400332).
Abstract
Fracture non-union occurs due to various factors, leading to the development of potentially substantial bone defects. While autograft and allograft are the current gold standards for non-union fractures, challenges related to availability and immune rejection highlight the need for improved treatments. A strategy in bone tissue engineering is to harness growth factors to induce an effect on cells to change their phenotype, behavior and initiate signaling pathways which lead to increased matrix deposition and tissue formation. Bone morphogenetic protein-2 (BMP-2) is a potent osteogenic growth factor however, given its rapid clearance time in vivo, there is a specific therapeutic window for efficacy while avoiding potential deleterious side-effects. It is demonstrated that a Laponite nanoclay coating on a 3D printable and bioresorbable poly(caprolactone) trimethacrylate-based resin enables binding of BMP-2, decreases the rate of release, enabling reduced concentrations to be used while enhancing osteoinduction in both in vitro and in vivo models.
Text
Adv Materials Inter - 2024 - Marshall - Using Laponite to Deliver BMP‐2 for Bone Tissue Engineering In Vitro
- Version of Record
More information
Accepted/In Press date: 16 July 2024
e-pub ahead of print date: 29 July 2024
Published date: 29 July 2024
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Publisher Copyright:
© 2024 The Author(s). Advanced Materials Interfaces published by Wiley-VCH GmbH.
Keywords:
biomaterial, bone tissue engineering, CAM assay, Laponite, subcutaneous implantation model
Identifiers
Local EPrints ID: 494032
URI: http://eprints.soton.ac.uk/id/eprint/494032
PURE UUID: 5be2af57-a2ae-416d-9fe7-dae3991fbbd6
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Date deposited: 19 Sep 2024 16:57
Last modified: 16 Oct 2024 02:01
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Contributors
Author:
Karen M. Marshall
Author:
Jonathan P. Wojciechowski
Author:
Cécile Echalier
Author:
Sebastien J.P. Callens
Author:
Tao Yang
Author:
Øystein Øvrebø
Author:
Kun Zhou
Author:
Vineetha Jayawarna
Author:
Janos M. Kanczler
Author:
Molly M. Stevens
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