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Exploring the causal effect of maternal pregnancy adiposity on offspring adiposity: Mendelian randomisation using polygenic risk scores

Exploring the causal effect of maternal pregnancy adiposity on offspring adiposity: Mendelian randomisation using polygenic risk scores
Exploring the causal effect of maternal pregnancy adiposity on offspring adiposity: Mendelian randomisation using polygenic risk scores
Background: greater maternal adiposity before or during pregnancy is associated with greater offspring adiposity throughout childhood, but the extent to which this is due to causal intrauterine or periconceptional mechanisms remains unclear. Here, we use Mendelian randomisation (MR) with polygenic risk scores (PRS) to investigate whether associations between maternal pre-/early pregnancy body mass index (BMI) and offspring adiposity from birth to adolescence are causal.

Methods: we undertook confounder adjusted multivariable (MV) regression and MR using mother-offspring pairs from two UK cohorts: Avon Longitudinal Study of Parents and Children (ALSPAC) and Born in Bradford (BiB). In ALSPAC and BiB, the outcomes were birthweight (BW; N = 9339) and BMI at age 1 and 4 years (N = 8659 to 7575). In ALSPAC only we investigated BMI at 10 and 15 years (N = 4476 to 4112) and dual-energy X-ray absorptiometry (DXA) determined fat mass index (FMI) from age 10–18 years (N = 2659 to 3855). We compared MR results from several PRS, calculated from maternal non-transmitted alleles at between 29 and 80,939 single nucleotide polymorphisms (SNPs).

Results: MV and MR consistently showed a positive association between maternal BMI and BW, supporting a moderate causal effect. For adiposity at most older ages, although MV estimates indicated a strong positive association, MR estimates did not support a causal effect. For the PRS with few SNPs, MR estimates were statistically consistent with the null, but had wide confidence intervals so were often also statistically consistent with the MV estimates. In contrast, the largest PRS yielded MR estimates with narrower confidence intervals, providing strong evidence that the true causal effect on adolescent adiposity is smaller than the MV estimates (Pdifference = 0.001 for 15-year BMI). This suggests that the MV estimates are affected by residual confounding, therefore do not provide an accurate indication of the causal effect size.

Conclusions: our results suggest that higher maternal pre-/early-pregnancy BMI is not a key driver of higher adiposity in the next generation. Thus, they support interventions that target the whole population for reducing overweight and obesity, rather than a specific focus on women of reproductive age.
1741-7015
Bond, Tom A.
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Richmond, Rebecca C.
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Karhunen, Ville
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Cuellar-Partida, Gabriel
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Borges, Maria Carolina
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Zuber, Verena
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Couto Alves, Alexessander
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Mason, Dan
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Yang, Tiffany C.
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Gunter, Marc J.
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Dehghan, Abbas
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Tzoulaki, Ioanna
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Sebert, Sylvain
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Evans, David M.
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Lewin, Alex M.
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O’Reilly, Paul F.
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Lawlor, Deborah A.
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Järvelin, Marjo-Ritter
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Bond, Tom A.
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Richmond, Rebecca C.
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Karhunen, Ville
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Cuellar-Partida, Gabriel
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Borges, Maria Carolina
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Zuber, Verena
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Couto Alves, Alexessander
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Mason, Dan
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Yang, Tiffany C.
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Gunter, Marc J.
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Dehghan, Abbas
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Tzoulaki, Ioanna
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Sebert, Sylvain
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Evans, David M.
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Lewin, Alex M.
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O’Reilly, Paul F.
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Lawlor, Deborah A.
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Järvelin, Marjo-Ritter
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Bond, Tom A., Richmond, Rebecca C., Karhunen, Ville, Cuellar-Partida, Gabriel, Borges, Maria Carolina, Zuber, Verena, Couto Alves, Alexessander, Mason, Dan, Yang, Tiffany C., Gunter, Marc J., Dehghan, Abbas, Tzoulaki, Ioanna, Sebert, Sylvain, Evans, David M., Lewin, Alex M., O’Reilly, Paul F., Lawlor, Deborah A. and Järvelin, Marjo-Ritter (2022) Exploring the causal effect of maternal pregnancy adiposity on offspring adiposity: Mendelian randomisation using polygenic risk scores. BMC Medicine, 20, [34]. (doi:10.1186/s12916-021-02216-w).

Record type: Article

Abstract

Background: greater maternal adiposity before or during pregnancy is associated with greater offspring adiposity throughout childhood, but the extent to which this is due to causal intrauterine or periconceptional mechanisms remains unclear. Here, we use Mendelian randomisation (MR) with polygenic risk scores (PRS) to investigate whether associations between maternal pre-/early pregnancy body mass index (BMI) and offspring adiposity from birth to adolescence are causal.

Methods: we undertook confounder adjusted multivariable (MV) regression and MR using mother-offspring pairs from two UK cohorts: Avon Longitudinal Study of Parents and Children (ALSPAC) and Born in Bradford (BiB). In ALSPAC and BiB, the outcomes were birthweight (BW; N = 9339) and BMI at age 1 and 4 years (N = 8659 to 7575). In ALSPAC only we investigated BMI at 10 and 15 years (N = 4476 to 4112) and dual-energy X-ray absorptiometry (DXA) determined fat mass index (FMI) from age 10–18 years (N = 2659 to 3855). We compared MR results from several PRS, calculated from maternal non-transmitted alleles at between 29 and 80,939 single nucleotide polymorphisms (SNPs).

Results: MV and MR consistently showed a positive association between maternal BMI and BW, supporting a moderate causal effect. For adiposity at most older ages, although MV estimates indicated a strong positive association, MR estimates did not support a causal effect. For the PRS with few SNPs, MR estimates were statistically consistent with the null, but had wide confidence intervals so were often also statistically consistent with the MV estimates. In contrast, the largest PRS yielded MR estimates with narrower confidence intervals, providing strong evidence that the true causal effect on adolescent adiposity is smaller than the MV estimates (Pdifference = 0.001 for 15-year BMI). This suggests that the MV estimates are affected by residual confounding, therefore do not provide an accurate indication of the causal effect size.

Conclusions: our results suggest that higher maternal pre-/early-pregnancy BMI is not a key driver of higher adiposity in the next generation. Thus, they support interventions that target the whole population for reducing overweight and obesity, rather than a specific focus on women of reproductive age.

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Accepted/In Press date: 13 December 2021
Published date: 1 February 2022

Identifiers

Local EPrints ID: 494095
URI: http://eprints.soton.ac.uk/id/eprint/494095
ISSN: 1741-7015
PURE UUID: 6a4e192b-beae-4644-847b-79b2f4675dd2
ORCID for Alexessander Couto Alves: ORCID iD orcid.org/0000-0001-8519-7356

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Date deposited: 24 Sep 2024 16:30
Last modified: 01 Oct 2024 02:14

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Contributors

Author: Tom A. Bond
Author: Rebecca C. Richmond
Author: Ville Karhunen
Author: Gabriel Cuellar-Partida
Author: Maria Carolina Borges
Author: Verena Zuber
Author: Alexessander Couto Alves ORCID iD
Author: Dan Mason
Author: Tiffany C. Yang
Author: Marc J. Gunter
Author: Abbas Dehghan
Author: Ioanna Tzoulaki
Author: Sylvain Sebert
Author: David M. Evans
Author: Alex M. Lewin
Author: Paul F. O’Reilly
Author: Deborah A. Lawlor
Author: Marjo-Ritter Järvelin

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