Gerard, Matthew D., Cook, Hiroki Marius Oswald, Read, James A., Abughazaleh, Ibrahim H., Srisamran, Panuwat, Chugh, Siddhi, Liang, Sijing, Fu, Qiang, Oreffo, Richard O.C., Richardson, David J., Mahajan, Sumeet and Xu, Lin (2024) Single-cell high-precision ablation using nanosecond-pulsed thulium-doped fiber laser. Optical Engineering, 63 (8), [086102]. (doi:10.1117/1.OE.63.8.086102).
Abstract
Significance: precise laser ablation at the single-cell level is demonstrated on onion epidermal and human neuroblastoma cells for the first time using a 1.95 μm thuliumdoped fiber laser (TDFL). The high-precision laser ablation demonstrated paves the way for micro-dissection and surgery in scientific and clinical applications.
Aim: we aimed to ablate individual target cells with pulsed laser radiation while minimizing damage to neighboring cells and to study the effects of pulse variation on laser ablation outcome.
Approach: an advanced 1.95 μm nanosecond-pulsed TDFL is developed offering a high degree of pulse control and coupled to a microscope to enable simultaneous ablating and monitoring. A reflective microscope objective is used to focus the light onto the sample without chromatic aberrations. Systematic studies of ablation outcomes using different pulse parameters are then performed.
Results: single-cell ablation is achieved, with a precision of 31.3 0.1 μm for onion epidermal cells and 20 μm for fix
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