READ ME File For 'Single-cell electro-mechanical shear flow deformability cytometry'

Dataset DOI: https://doi.org/10.5258/SOTON/D3151

ReadMe Author: Junyu Chen, University of Southampton, https://orcid.org/my-orcid?orcid=0009-0006-9975-7493

This dataset supports the publication:
AUTHORS: Junyu Chen 
CONTRIBUTORS:Xueping Zou, Daniel C. Spencer and Hywel Morgan.
TITLE: Single-cell electro-mechanical shear flow deformability cytometry
JOURNAL: Nature Microsystems and Nanoengineering


This dataset contains data for the figures

The figures are as follows:
Figure 1  Density plots of electrical deformability (c) and electrical opacity (d) as a function of electrical diameter for HL60 cells
Figure 2  Figure 2 (c) Sensitivity plots for vertical impedance Z1 and (d) lateral impedance Z2 as a function of the deformation index (major/minor axis) for three different sizes of particles. (e) Electrical deformability (Z1/Z2) as a function of the deformation index for three different sizes of particles.
Figure 3  Osmotic data，information on the data is contained in the first sheet (named "Information")
Figure 4  GA fixation data，information on the data is contained in the first sheet (named "Information")
Figure 5  CytoD data，information on the data is contained in the first sheet (named "Information")
Figure 6  Correlation between the Electrical Deformability (ED) and Optical Deformability (OD) for HL60 cells (Control, Glutaraldehyde (GA) and CytoD treated) together with beads. 

Supplementary Figure 2  (b) Magnitude of the vertical impedance Z1 (red) and lateral impedance Z2 (blue) as a function of vertical position in the channel (z-axis) for a 5µm diameter particle. (c) as for (b) but for a 12µm diameter particle. 
Supplementary Figure 3  (b) Histogram showing the experimentally measured velocities of a mixture of 5 µm and 10µm diameter beads (suspended in PBS) at a volumetric flow rate of 5µl/min (approximately 3,000 particles); (c) Histogram for the same particles suspended in 0.5%v/v MC-PBS at a flow rate of 5µl/min.
Supplementary Figure 4 (a). Electrical deformability of GA fixed cell versus electrical diameter at a flow rate of 10μl/min. (b) Dose-response curve and Hill equation fit for electrical deformability at a flow rate of 10μl/min giving EC50 = 0.0012% v/v.
Supplementary Figure 5 (a). Electrical deformability of CytoD treated HL60 cells at a flow rate of 10μl/min. (b) Dose-response plot of electrical deformability at a flow rate of 10μl/min giving EC50 = 11nM.


Date that the file was created: 27th June 2024