A combined electro-optical deformability micro-cytometer
A combined electro-optical deformability micro-cytometer
We have developed a deformability cytometer that simultaneously measures the optical and electrical shape change of single cells in a viscoelastic shear flow. The optical deformability of single cells is measured using a low-cost CMOS camera illuminated with a high-power LED triggered from an electrical impedance signal created by a passing cell. Simultaneously the electrical deformability of the cell is determined using electrode arrays that measure shape changes along different axes. This is achieved by correlating the optical and electrical signals captured without complicated synchronisation. The system was characterised by measuring the deformability of HL-60 cells treated with cytochalasin D, latrunculin B and glutaraldehyde. Results demonstrate excellent correlation between the optical and electrical methods.
34270-34278
Zou, Xueping
4092b1ea-004f-44b2-a296-09a559dcd268
Spencer, Daniel C.
4affe9f6-353a-4507-8066-0180b8dc9eaf
Chen, Junyu
2b134f76-80fa-40b6-aa53-09ff5be70c58
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Zou, Xueping
4092b1ea-004f-44b2-a296-09a559dcd268
Spencer, Daniel C.
4affe9f6-353a-4507-8066-0180b8dc9eaf
Chen, Junyu
2b134f76-80fa-40b6-aa53-09ff5be70c58
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Zou, Xueping, Spencer, Daniel C., Chen, Junyu and Morgan, Hywel
(2024)
A combined electro-optical deformability micro-cytometer.
RSC Advances, 2024 (46), .
(doi:10.1039/D4RA04800H).
Abstract
We have developed a deformability cytometer that simultaneously measures the optical and electrical shape change of single cells in a viscoelastic shear flow. The optical deformability of single cells is measured using a low-cost CMOS camera illuminated with a high-power LED triggered from an electrical impedance signal created by a passing cell. Simultaneously the electrical deformability of the cell is determined using electrode arrays that measure shape changes along different axes. This is achieved by correlating the optical and electrical signals captured without complicated synchronisation. The system was characterised by measuring the deformability of HL-60 cells treated with cytochalasin D, latrunculin B and glutaraldehyde. Results demonstrate excellent correlation between the optical and electrical methods.
Text
d4ra04800h
- Version of Record
More information
Accepted/In Press date: 22 October 2024
e-pub ahead of print date: 29 October 2024
Identifiers
Local EPrints ID: 495931
URI: http://eprints.soton.ac.uk/id/eprint/495931
ISSN: 2046-2069
PURE UUID: de2704e1-e67a-4bae-bc8c-6ba67d4a9f6d
Catalogue record
Date deposited: 27 Nov 2024 17:56
Last modified: 28 Nov 2024 03:08
Export record
Altmetrics
Contributors
Author:
Xueping Zou
Author:
Daniel C. Spencer
Author:
Junyu Chen
Author:
Hywel Morgan
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics