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Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton

Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton
Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton
We investigated the ability of four species of marine zooplankton to synthesize polyunsaturated fatty acids (PUFAs) during pulse-chase tracer experiments. Liposomes containing a deuterium labelled precursor fatty acid, D5-18:3n-3, were fed to female Calanus finmarchicus, Calanoides acutus, Drepanopus forcipatus and calyptopus larvae of Euphausia superba during ship-board experiments. Although all species of zooplankton readily ingested the liposomes and incorporated the D5-18:3n-3 tracer into their somatic lipid pool, only negligible products of elongation and desaturation; D5-18:4n-3, D5-20:5n-3 and D5-22:6n-3 were detected after 96 h incubations. We conclude that the four species of marine zooplankton examined here are unable to synthesize PUFA at ecologically significant rates and certainly not in amounts sufficient to support growth and reproductive processes.
0142-7873
417-422
Bell, M.V.
a79eb3af-ce74-40ee-826c-eb4586b22f59
Dick, J.R.
2c2868f3-6a78-4c7f-8a28-b4be8dd0559a
Anderson, T.R.
dfed062f-e747-48d3-b59e-2f5e57a8571d
Pond, D.W.
1997312b-b198-4a52-89e0-e7bfc5aa9250
Bell, M.V.
a79eb3af-ce74-40ee-826c-eb4586b22f59
Dick, J.R.
2c2868f3-6a78-4c7f-8a28-b4be8dd0559a
Anderson, T.R.
dfed062f-e747-48d3-b59e-2f5e57a8571d
Pond, D.W.
1997312b-b198-4a52-89e0-e7bfc5aa9250

Bell, M.V., Dick, J.R., Anderson, T.R. and Pond, D.W. (1970) Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton. Journal of Plankton Research, 29 (5), 417-422. (doi:10.1093/plankt/fbm025).

Record type: Article

Abstract

We investigated the ability of four species of marine zooplankton to synthesize polyunsaturated fatty acids (PUFAs) during pulse-chase tracer experiments. Liposomes containing a deuterium labelled precursor fatty acid, D5-18:3n-3, were fed to female Calanus finmarchicus, Calanoides acutus, Drepanopus forcipatus and calyptopus larvae of Euphausia superba during ship-board experiments. Although all species of zooplankton readily ingested the liposomes and incorporated the D5-18:3n-3 tracer into their somatic lipid pool, only negligible products of elongation and desaturation; D5-18:4n-3, D5-20:5n-3 and D5-22:6n-3 were detected after 96 h incubations. We conclude that the four species of marine zooplankton examined here are unable to synthesize PUFA at ecologically significant rates and certainly not in amounts sufficient to support growth and reproductive processes.

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Published date: 1 January 1970

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Local EPrints ID: 49662
URI: http://eprints.soton.ac.uk/id/eprint/49662
ISSN: 0142-7873
PURE UUID: bc5c1b9d-8455-4a6d-9ccf-f7a436c91454

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Date deposited: 20 Nov 2007
Last modified: 13 Mar 2019 20:53

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Contributors

Author: M.V. Bell
Author: J.R. Dick
Author: T.R. Anderson
Author: D.W. Pond

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