The University of Southampton
University of Southampton Institutional Repository

Clay nanofiller enhances and stabilises a new injectable human bone extracellular matrix scaffold for skeletal regeneration

Clay nanofiller enhances and stabilises a new injectable human bone extracellular matrix scaffold for skeletal regeneration
Clay nanofiller enhances and stabilises a new injectable human bone extracellular matrix scaffold for skeletal regeneration
Critical bone defects and fractures are typically treated using autologous bone grafts, which are limited by volume of bone that can be harvested, or allogeneic or synthetic bone grafts that lack osteoinductive properties. Human bone extracellular matrix (hbECM) is widely available and offers the potential to be used as a native material to synthesize functional injectable hydrogel systems. However, hbECM lacks the mechanical stability required for injectability and bone growth. We have explored the use of Laponite® (LAP) nanoclay and sodium polyacrylate to augment the mechanical and biological properties of hbECM gel. We demonstrated that the inclusion of LAP into ECM improved the physicochemical properties of hbECM and consequently promoted cell responses confirming that the nanoclay platelet-to-platelet interaction is key to sustain hbECM functionality. This novel hbECM detailed offers significant clinical promise for bone repair.
Bone, Decellularization, Hydrogel, Injectable, Nanocomposite
2352-4928
Cidonio, Gianluca
558ad583-899a-4d8c-b42b-bc1c354c8757
Dodhia, Vikash H.
ce7fa7cd-05fa-4e0b-a43c-45482c17d812
Iafrate, Lucia
13dcca9c-ad09-4479-adf9-aaf9ee59cc09
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Rau, Julietta V.
0e2be159-d836-49ef-823a-a00f0452c080
Giliberti, Valeria
7389de21-cf4c-4e3f-b81f-943708af85be
Nucara, Alessandro
6060c3fe-dad5-40e6-be8e-0b07299f9343
Oreffo, Richard O.C.
8f43702d-637e-45f9-b374-3d01c145b16c
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
Kim, Yang-Hee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Cidonio, Gianluca
558ad583-899a-4d8c-b42b-bc1c354c8757
Dodhia, Vikash H.
ce7fa7cd-05fa-4e0b-a43c-45482c17d812
Iafrate, Lucia
13dcca9c-ad09-4479-adf9-aaf9ee59cc09
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Rau, Julietta V.
0e2be159-d836-49ef-823a-a00f0452c080
Giliberti, Valeria
7389de21-cf4c-4e3f-b81f-943708af85be
Nucara, Alessandro
6060c3fe-dad5-40e6-be8e-0b07299f9343
Oreffo, Richard O.C.
8f43702d-637e-45f9-b374-3d01c145b16c
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
Kim, Yang-Hee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d

Cidonio, Gianluca, Dodhia, Vikash H., Iafrate, Lucia, Kanczler, Janos M., Rau, Julietta V., Giliberti, Valeria, Nucara, Alessandro, Oreffo, Richard O.C., Dawson, Jonathan I. and Kim, Yang-Hee (2024) Clay nanofiller enhances and stabilises a new injectable human bone extracellular matrix scaffold for skeletal regeneration. Materials Today Communications, 39, [109082]. (doi:10.1016/j.mtcomm.2024.109082).

Record type: Article

Abstract

Critical bone defects and fractures are typically treated using autologous bone grafts, which are limited by volume of bone that can be harvested, or allogeneic or synthetic bone grafts that lack osteoinductive properties. Human bone extracellular matrix (hbECM) is widely available and offers the potential to be used as a native material to synthesize functional injectable hydrogel systems. However, hbECM lacks the mechanical stability required for injectability and bone growth. We have explored the use of Laponite® (LAP) nanoclay and sodium polyacrylate to augment the mechanical and biological properties of hbECM gel. We demonstrated that the inclusion of LAP into ECM improved the physicochemical properties of hbECM and consequently promoted cell responses confirming that the nanoclay platelet-to-platelet interaction is key to sustain hbECM functionality. This novel hbECM detailed offers significant clinical promise for bone repair.

Text
MTCOMM-D-23-12612_R1 - Accepted Manuscript
Restricted to Repository staff only
Request a copy
Text
1-s2.0-S2352492824010638-main - Version of Record
Available under License Creative Commons Attribution.
Download (5MB)

More information

Accepted/In Press date: 29 April 2024
e-pub ahead of print date: 30 April 2024
Published date: 11 May 2024
Keywords: Bone, Decellularization, Hydrogel, Injectable, Nanocomposite

Identifiers

Local EPrints ID: 497041
URI: http://eprints.soton.ac.uk/id/eprint/497041
ISSN: 2352-4928
PURE UUID: ea1ff11c-6c3f-4394-84b6-7aaed88d2387
ORCID for Janos M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Jonathan I. Dawson: ORCID iD orcid.org/0000-0002-6712-0598
ORCID for Yang-Hee Kim: ORCID iD orcid.org/0000-0002-5312-3448

Catalogue record

Date deposited: 10 Jan 2025 17:46
Last modified: 22 Aug 2025 02:14

Export record

Altmetrics

Contributors

Author: Gianluca Cidonio
Author: Vikash H. Dodhia
Author: Lucia Iafrate
Author: Janos M. Kanczler ORCID iD
Author: Julietta V. Rau
Author: Valeria Giliberti
Author: Alessandro Nucara
Author: Richard O.C. Oreffo
Author: Yang-Hee Kim ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×