The University of Southampton
University of Southampton Institutional Repository

Design and construction of a micro-milled fluidic device as part of a DNA biosensor

Design and construction of a micro-milled fluidic device as part of a DNA biosensor
Design and construction of a micro-milled fluidic device as part of a DNA biosensor
Under the Optonanogen project (EU contract IST-2001237239), a novel biosensor has been developed, which incorporates a disposable acrylic (PMMA) fluidic header [1]. This biosensor relies on a micro-cantilever array which is etched into a silicon chip and contained within the header. Nucleic acids are immobilised on one side of each of the 20 cantilevers, then upon injection of a biological sample over the cantilevers, hybridisation of DNA contained within the sample (and complementary to the immobilised nucleic acids) generates surface stresses on the cantilever and causes bending. The cantilever deflection is then detected optically using laser and micro-lens arrays. The sensor was initially designed to detect human gene mutations, particularly those associated with breast cancer, and miniaturises this form of diagnostic test [2]. This paper, however, concentrates on the fluidic design of the header, its ease of use and the ability to deliver the test sample to the cantilevers.
0954-4062
847-853
Townsend, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wenn, D.
700410f9-ffca-427d-ad7a-836ba5529409
Brennan, D.
b6a79239-f3d3-4637-a3b9-e2fd2190344a
Grabham, N.J.
00695728-6280-4d06-a943-29142f2547c9
Townsend, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wenn, D.
700410f9-ffca-427d-ad7a-836ba5529409
Brennan, D.
b6a79239-f3d3-4637-a3b9-e2fd2190344a
Grabham, N.J.
00695728-6280-4d06-a943-29142f2547c9

Townsend, R.J., Harris, N.R., Wenn, D., Brennan, D. and Grabham, N.J. (2008) Design and construction of a micro-milled fluidic device as part of a DNA biosensor. Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science, 222, 847-853. (doi:10.1243/09544062JMES715).

Record type: Article

Abstract

Under the Optonanogen project (EU contract IST-2001237239), a novel biosensor has been developed, which incorporates a disposable acrylic (PMMA) fluidic header [1]. This biosensor relies on a micro-cantilever array which is etched into a silicon chip and contained within the header. Nucleic acids are immobilised on one side of each of the 20 cantilevers, then upon injection of a biological sample over the cantilevers, hybridisation of DNA contained within the sample (and complementary to the immobilised nucleic acids) generates surface stresses on the cantilever and causes bending. The cantilever deflection is then detected optically using laser and micro-lens arrays. The sensor was initially designed to detect human gene mutations, particularly those associated with breast cancer, and miniaturises this form of diagnostic test [2]. This paper, however, concentrates on the fluidic design of the header, its ease of use and the ability to deliver the test sample to the cantilevers.

Text
Townsend_et_al.pdf - Other
Download (391kB)

More information

Submitted date: 7 August 2007
Published date: 2008

Identifiers

Local EPrints ID: 49716
URI: http://eprints.soton.ac.uk/id/eprint/49716
ISSN: 0954-4062
PURE UUID: 4f333f01-5194-44e9-be45-bec2a2b7ffb0
ORCID for N.R. Harris: ORCID iD orcid.org/0000-0003-4122-2219
ORCID for N.J. Grabham: ORCID iD orcid.org/0000-0002-6385-0331

Catalogue record

Date deposited: 26 Nov 2007
Last modified: 16 Mar 2024 02:45

Export record

Altmetrics

Contributors

Author: R.J. Townsend
Author: N.R. Harris ORCID iD
Author: D. Wenn
Author: D. Brennan
Author: N.J. Grabham ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×