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Design and construction of a micro-milled fluidic device as part of a DNA biosensor

Design and construction of a micro-milled fluidic device as part of a DNA biosensor
Design and construction of a micro-milled fluidic device as part of a DNA biosensor
Under the Optonanogen project (EU contract IST-2001237239), a novel biosensor has been developed, which incorporates a disposable acrylic (PMMA) fluidic header [1]. This biosensor relies on a micro-cantilever array which is etched into a silicon chip and contained within the header. Nucleic acids are immobilised on one side of each of the 20 cantilevers, then upon injection of a biological sample over the cantilevers, hybridisation of DNA contained within the sample (and complementary to the immobilised nucleic acids) generates surface stresses on the cantilever and causes bending. The cantilever deflection is then detected optically using laser and micro-lens arrays. The sensor was initially designed to detect human gene mutations, particularly those associated with breast cancer, and miniaturises this form of diagnostic test [2]. This paper, however, concentrates on the fluidic design of the header, its ease of use and the ability to deliver the test sample to the cantilevers.
0954-4062
847-853
Townsend, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wenn, D.
700410f9-ffca-427d-ad7a-836ba5529409
Brennan, D.
b6a79239-f3d3-4637-a3b9-e2fd2190344a
Grabham, N.J.
00695728-6280-4d06-a943-29142f2547c9
Townsend, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Wenn, D.
700410f9-ffca-427d-ad7a-836ba5529409
Brennan, D.
b6a79239-f3d3-4637-a3b9-e2fd2190344a
Grabham, N.J.
00695728-6280-4d06-a943-29142f2547c9

Townsend, R.J., Harris, N.R., Wenn, D., Brennan, D. and Grabham, N.J. (2008) Design and construction of a micro-milled fluidic device as part of a DNA biosensor Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science, 222, pp. 847-853. (doi:10.1243/09544062JMES715).

Record type: Article

Abstract

Under the Optonanogen project (EU contract IST-2001237239), a novel biosensor has been developed, which incorporates a disposable acrylic (PMMA) fluidic header [1]. This biosensor relies on a micro-cantilever array which is etched into a silicon chip and contained within the header. Nucleic acids are immobilised on one side of each of the 20 cantilevers, then upon injection of a biological sample over the cantilevers, hybridisation of DNA contained within the sample (and complementary to the immobilised nucleic acids) generates surface stresses on the cantilever and causes bending. The cantilever deflection is then detected optically using laser and micro-lens arrays. The sensor was initially designed to detect human gene mutations, particularly those associated with breast cancer, and miniaturises this form of diagnostic test [2]. This paper, however, concentrates on the fluidic design of the header, its ease of use and the ability to deliver the test sample to the cantilevers.

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Submitted date: 7 August 2007
Published date: 2008

Identifiers

Local EPrints ID: 49716
URI: http://eprints.soton.ac.uk/id/eprint/49716
ISSN: 0954-4062
PURE UUID: 4f333f01-5194-44e9-be45-bec2a2b7ffb0
ORCID for N.R. Harris: ORCID iD orcid.org/0000-0003-4122-2219
ORCID for N.J. Grabham: ORCID iD orcid.org/0000-0002-6385-0331

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Date deposited: 26 Nov 2007
Last modified: 06 Sep 2017 16:32

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Contributors

Author: R.J. Townsend
Author: N.R. Harris ORCID iD
Author: D. Wenn
Author: D. Brennan
Author: N.J. Grabham ORCID iD

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