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Single-cell electro-mechanical cytometry

Single-cell electro-mechanical cytometry
Single-cell electro-mechanical cytometry
The complex structural and molecular features of a cell lead to a set of specific dielectric and mechanical properties which can serve as intrinsic phenotypic markers that enable different cell populations to be characterised and distinguished. We have developed a novel microfluidic technique that simultaneously measures both the electrical and mechanical properties of single cells at high speed. Cells flow along a microchannel and are deformed (elongated) to different degrees by the shear force created by a viscoelastic fluid and channel wall. The electrical impedance of each cell is measured along orthogonal axes to determine the shape change and thus the electrical deformability, along with cell dielectric properties. The system performance was evaluated by measuring the electro-mechanical properties of cells treated in different ways, including osmotic shock, glutaraldehyde cross-linking and cytoskeletal disruption with cytochalasin D and latrunculin B. This novel cytometer has a throughput of ~ 100 cells s− 1 is simple, and does not require sheath flow or rely on high speed optical imaging.
Research Square
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Chen, Junyu
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Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Chen, Junyu
2b134f76-80fa-40b6-aa53-09ff5be70c58

[Unknown type: UNSPECIFIED]

Record type: UNSPECIFIED

Abstract

The complex structural and molecular features of a cell lead to a set of specific dielectric and mechanical properties which can serve as intrinsic phenotypic markers that enable different cell populations to be characterised and distinguished. We have developed a novel microfluidic technique that simultaneously measures both the electrical and mechanical properties of single cells at high speed. Cells flow along a microchannel and are deformed (elongated) to different degrees by the shear force created by a viscoelastic fluid and channel wall. The electrical impedance of each cell is measured along orthogonal axes to determine the shape change and thus the electrical deformability, along with cell dielectric properties. The system performance was evaluated by measuring the electro-mechanical properties of cells treated in different ways, including osmotic shock, glutaraldehyde cross-linking and cytoskeletal disruption with cytochalasin D and latrunculin B. This novel cytometer has a throughput of ~ 100 cells s− 1 is simple, and does not require sheath flow or rely on high speed optical imaging.

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Submitted date: 16 February 2024
Published date: 22 November 2024

Identifiers

Local EPrints ID: 497176
URI: http://eprints.soton.ac.uk/id/eprint/497176
PURE UUID: cbbf68ea-94d1-441e-982d-d589501e5d79
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Junyu Chen: ORCID iD orcid.org/0009-0006-9975-7493

Catalogue record

Date deposited: 15 Jan 2025 17:57
Last modified: 22 Aug 2025 02:43

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Contributors

Author: Hywel Morgan ORCID iD
Author: Daniel Spencer
Author: Junyu Chen ORCID iD

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