Electrochemical detection of non-esterified fatty acid by layer-by-layer assembled enzyme electrodes
Electrochemical detection of non-esterified fatty acid by layer-by-layer assembled enzyme electrodes
In this study, detection and measurement of non-esterified fatty acids (NEFA) concentration has been achieved by electrochemical method in one operation step. Multilayer films of poly(dimethyldiallyammonium chloride) (PDA) wrapped multi-wall carbon nanotubes (MWCNTs) and two enzymes acyl-CoA synthetase (ACS) and acyl-CoA oxidase (ACOD) were assembled on a carbon screen printed electrode by the layer-by-layer (LbL) immobilization. The fine polymer-enzyme layers produced by the LbL method, allowed mass transport from the reactant cascading down the layers to accomplish the two-step enzyme reactions. The polymer-CNTs and enzyme modified electrode exhibited good electrocatalytical property retaining enzyme activity. Linear increase of anodic current from H2O2 produced from NEFA oxidation was observed with the increasing concentrations of oleic acid. These results indicate a promising technique for a simple, rapid one-step determination of NEFA for diabetes management.
Biosensor, Diabetes, Enzyme reaction, Layer-by-layer assembly, Non-esterified fatty acids, Poly(dimethyldiallyammonium chloride)
535-541
Kang, Jing
bb70b0d1-bfbf-4ea5-b77e-24ab6f3bab63
Hussain, Anisah T.
b4bdcc3a-689e-4f1a-a4f7-e48c7b490541
Catt, Michael
1235d6a3-9c23-45e9-ae2b-213c9e6175c5
Trenell, Michael
4a4e3364-e3f3-4504-85a0-9c039c09d871
Haggett, Barry
1054c046-e5e3-4a81-8a9a-3ba8abd92a6b
Yu, Eileen Hao
28e47863-4b50-4821-b80b-71fb5a2edef2
2014
Kang, Jing
bb70b0d1-bfbf-4ea5-b77e-24ab6f3bab63
Hussain, Anisah T.
b4bdcc3a-689e-4f1a-a4f7-e48c7b490541
Catt, Michael
1235d6a3-9c23-45e9-ae2b-213c9e6175c5
Trenell, Michael
4a4e3364-e3f3-4504-85a0-9c039c09d871
Haggett, Barry
1054c046-e5e3-4a81-8a9a-3ba8abd92a6b
Yu, Eileen Hao
28e47863-4b50-4821-b80b-71fb5a2edef2
Kang, Jing, Hussain, Anisah T., Catt, Michael, Trenell, Michael, Haggett, Barry and Yu, Eileen Hao
(2014)
Electrochemical detection of non-esterified fatty acid by layer-by-layer assembled enzyme electrodes.
Sensors and Actuators, B: Chemical, 190, .
(doi:10.1016/j.snb.2013.09.011).
Abstract
In this study, detection and measurement of non-esterified fatty acids (NEFA) concentration has been achieved by electrochemical method in one operation step. Multilayer films of poly(dimethyldiallyammonium chloride) (PDA) wrapped multi-wall carbon nanotubes (MWCNTs) and two enzymes acyl-CoA synthetase (ACS) and acyl-CoA oxidase (ACOD) were assembled on a carbon screen printed electrode by the layer-by-layer (LbL) immobilization. The fine polymer-enzyme layers produced by the LbL method, allowed mass transport from the reactant cascading down the layers to accomplish the two-step enzyme reactions. The polymer-CNTs and enzyme modified electrode exhibited good electrocatalytical property retaining enzyme activity. Linear increase of anodic current from H2O2 produced from NEFA oxidation was observed with the increasing concentrations of oleic acid. These results indicate a promising technique for a simple, rapid one-step determination of NEFA for diabetes management.
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Published date: 2014
Keywords:
Biosensor, Diabetes, Enzyme reaction, Layer-by-layer assembly, Non-esterified fatty acids, Poly(dimethyldiallyammonium chloride)
Identifiers
Local EPrints ID: 498551
URI: http://eprints.soton.ac.uk/id/eprint/498551
ISSN: 0925-4005
PURE UUID: 0f015822-77af-4157-9cb4-d9528b00b69d
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Date deposited: 20 Feb 2025 18:16
Last modified: 22 Feb 2025 03:13
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Contributors
Author:
Jing Kang
Author:
Anisah T. Hussain
Author:
Michael Catt
Author:
Michael Trenell
Author:
Barry Haggett
Author:
Eileen Hao Yu
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