Numerical study of transient response in amperometric and potentiometric glucose biosensors
Numerical study of transient response in amperometric and potentiometric glucose biosensors
Biosensors are indispensable tools for continuous monitoring of key analytes, such as glucose, in chronic disease management. This thesis focuses on enzyme-immobilized redox polymer biosensors, leveraging reaction-diffusion partial differential equations (PDEs) to bridge the gap between theory and practical applications for biosensor design.
A transient numerical scheme in MATLAB was used to solve coupled non-linear PDEs, capturing the dynamic behavior of enzyme-membrane systems. Verification was achieved by comparing steady-state analytical solutions with numerical outputs, while Case diagrams were used to identify different kinetic and transport regimes. Experiments with deflavinated enzymes provided a means to validate our model by systematically manipulating key parameters. Simulation results were compared with experimental data, revealing key insights into transient enzyme-membrane interactions.
The study also investigated tubular flow electrodes, revealing the dependence of steady-state amperometric responses on solution viscosity under flow conditions. Experimentally obtained chronoamperograms were fitted to numerically solved dynamic simulations, confirming the model's predictive capabilities. Novel boundary conditions were also proposed to simulate potentiometric biosensors, broadening the scope of this work beyond amperometric sensing.
Animations visualizing concentration profiles and system responses offer a deeper understanding of transient and steady-state behaviors. These insights can contribute to the optimization of biosensor performance, including sensitivity, dynamic range, and response time, advancing the development of robust and accurate glucose monitoring technologies.
Khan, Muhammad Hashim
f4c4453b-98ac-431a-8877-71d501f19b00
2025
Khan, Muhammad Hashim
f4c4453b-98ac-431a-8877-71d501f19b00
Bartlett, Philip
d99446db-a59d-4f89-96eb-f64b5d8bb075
Denuault, Guy
5c76e69f-e04e-4be5-83c5-e729887ffd4e
Khan, Muhammad Hashim
(2025)
Numerical study of transient response in amperometric and potentiometric glucose biosensors.
University of Southampton, Doctoral Thesis, 247pp.
Record type:
Thesis
(Doctoral)
Abstract
Biosensors are indispensable tools for continuous monitoring of key analytes, such as glucose, in chronic disease management. This thesis focuses on enzyme-immobilized redox polymer biosensors, leveraging reaction-diffusion partial differential equations (PDEs) to bridge the gap between theory and practical applications for biosensor design.
A transient numerical scheme in MATLAB was used to solve coupled non-linear PDEs, capturing the dynamic behavior of enzyme-membrane systems. Verification was achieved by comparing steady-state analytical solutions with numerical outputs, while Case diagrams were used to identify different kinetic and transport regimes. Experiments with deflavinated enzymes provided a means to validate our model by systematically manipulating key parameters. Simulation results were compared with experimental data, revealing key insights into transient enzyme-membrane interactions.
The study also investigated tubular flow electrodes, revealing the dependence of steady-state amperometric responses on solution viscosity under flow conditions. Experimentally obtained chronoamperograms were fitted to numerically solved dynamic simulations, confirming the model's predictive capabilities. Novel boundary conditions were also proposed to simulate potentiometric biosensors, broadening the scope of this work beyond amperometric sensing.
Animations visualizing concentration profiles and system responses offer a deeper understanding of transient and steady-state behaviors. These insights can contribute to the optimization of biosensor performance, including sensitivity, dynamic range, and response time, advancing the development of robust and accurate glucose monitoring technologies.
Text
Hashim Khan Final Thesis
- Version of Record
Restricted to Repository staff only until 28 February 2026.
Text
Final-thesis-submission-Examination-Mr-Muhammad-Khan
Restricted to Repository staff only
More information
Submitted date: 2025
Published date: 2025
Identifiers
Local EPrints ID: 499278
URI: http://eprints.soton.ac.uk/id/eprint/499278
PURE UUID: d18db1de-1eb7-4746-b1f6-0682a25f70d1
Catalogue record
Date deposited: 13 Mar 2025 17:40
Last modified: 03 Jul 2025 02:27
Export record
Contributors
Author:
Muhammad Hashim Khan
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics