Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads
Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads
In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.
Markovic, Vladimir M.
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Tamás Bánsági, Jr
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McKenzie, Dennel
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Mai, Anthony
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Pojman, John A.
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Taylor, Annette F.
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26 March 2019
Markovic, Vladimir M.
f7bc7970-aacd-47e6-b97b-ed5fb371709e
Tamás Bánsági, Jr
3cd3efed-e968-4c7f-8717-a63753b01161
McKenzie, Dennel
d805095f-257f-4b66-b4c7-45adb0c22d02
Mai, Anthony
6c3cac1e-d93c-42b5-a454-f802f645670e
Pojman, John A.
d4ab5f67-2edf-48a0-bc95-39f7e10b9b52
Taylor, Annette F.
08028a29-428d-4732-b6b1-f7a93389b386
Markovic, Vladimir M., Tamás Bánsági, Jr, McKenzie, Dennel, Mai, Anthony, Pojman, John A. and Taylor, Annette F.
(2019)
Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads.
Chaos: An Interdisciplinary Journal of Nonlinear Science, 29 (3), [033130].
(doi:10.1063/1.5089295).
Abstract
In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.
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Accepted/In Press date: 6 March 2019
Published date: 26 March 2019
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Local EPrints ID: 499299
URI: http://eprints.soton.ac.uk/id/eprint/499299
ISSN: 1054-1500
PURE UUID: 9a6266c4-345f-49b5-a8b0-e5f2396b58cf
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Date deposited: 14 Mar 2025 17:37
Last modified: 15 Mar 2025 03:14
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Author:
Vladimir M. Markovic
Author:
Jr Tamás Bánsági
Author:
Dennel McKenzie
Author:
Anthony Mai
Author:
John A. Pojman
Author:
Annette F. Taylor
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