Collective behavior of urease pH clocks in nano- and microvesicles controlled by fast ammonia transport
Collective behavior of urease pH clocks in nano- and microvesicles controlled by fast ammonia transport
The transmission of chemical signals via an extracellular solution plays a vital role in collective behavior in cellular biological systems and may be exploited in applications of lipid vesicles such as drug delivery. Here, we investigated chemical communication in synthetic micro- and nanovesicles containing urease in a solution of urea and acid. We combined experiments with simulations to demonstrate that the fast transport of ammonia to the external solution governs the pH–time profile and synchronizes the timing of the pH clock reaction in a heterogeneous population of vesicles. This study shows how the rate of production and emission of a small basic product controls pH changes in active vesicles with a distribution of sizes and enzyme amounts, which may be useful in bioreactor or healthcare applications.
1979-1984
Miele, Ylenia
7dd284aa-6a92-4c48-8841-f63e1dbc1a9e
Jones, Stephen J.
5757fb8c-847f-48ac-a749-e12be9e913b1
Rossi, Federico
195c2796-4d83-4491-a1e0-4100db87a778
Beales, Paul A.
920bef12-e354-4ff6-a528-9cab2227f32e
Taylor, Annette F.
08028a29-428d-4732-b6b1-f7a93389b386
3 March 2022
Miele, Ylenia
7dd284aa-6a92-4c48-8841-f63e1dbc1a9e
Jones, Stephen J.
5757fb8c-847f-48ac-a749-e12be9e913b1
Rossi, Federico
195c2796-4d83-4491-a1e0-4100db87a778
Beales, Paul A.
920bef12-e354-4ff6-a528-9cab2227f32e
Taylor, Annette F.
08028a29-428d-4732-b6b1-f7a93389b386
Miele, Ylenia, Jones, Stephen J., Rossi, Federico, Beales, Paul A. and Taylor, Annette F.
(2022)
Collective behavior of urease pH clocks in nano- and microvesicles controlled by fast ammonia transport.
The Journal of Physical Chemistry Letters, 13 (8), .
(doi:10.1021/acs.jpclett.2c00069).
Abstract
The transmission of chemical signals via an extracellular solution plays a vital role in collective behavior in cellular biological systems and may be exploited in applications of lipid vesicles such as drug delivery. Here, we investigated chemical communication in synthetic micro- and nanovesicles containing urease in a solution of urea and acid. We combined experiments with simulations to demonstrate that the fast transport of ammonia to the external solution governs the pH–time profile and synchronizes the timing of the pH clock reaction in a heterogeneous population of vesicles. This study shows how the rate of production and emission of a small basic product controls pH changes in active vesicles with a distribution of sizes and enzyme amounts, which may be useful in bioreactor or healthcare applications.
Text
miele-et-al-2022-collective-behavior-of-urease-ph-clocks-in-nano-and-microvesicles-controlled-by-fast-ammonia-transport
- Version of Record
More information
Accepted/In Press date: 17 February 2022
e-pub ahead of print date: 21 February 2022
Published date: 3 March 2022
Identifiers
Local EPrints ID: 499425
URI: http://eprints.soton.ac.uk/id/eprint/499425
ISSN: 1948-7185
PURE UUID: 790e2bdb-9e5d-4718-8c65-2131009ab907
Catalogue record
Date deposited: 19 Mar 2025 17:45
Last modified: 20 Mar 2025 03:12
Export record
Altmetrics
Contributors
Author:
Ylenia Miele
Author:
Stephen J. Jones
Author:
Federico Rossi
Author:
Paul A. Beales
Author:
Annette F. Taylor
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics