Engineering enzyme-driven dynamic behaviour in lipid vesicles
Engineering enzyme-driven dynamic behaviour in lipid vesicles
The urea–urease system is a pH dependent enzymatic reaction that was proposed as a convenient model to study pH oscillations in vitro; here, in order to determine the best conditions for oscillations, a two-variable model is used in which acid and substrate, urea, are supplied at rates and from an external medium to an enzyme-containing compartment. Oscillations were observed between pH 4 and 8. Thus the reaction appears a good candidate for the observation of oscillations in experiments, providing the necessary condition that is met. In order to match these conditions, we devised an experimental system where we can ensure the fast transport of acid to the encapsulated urease, compared to that of urea. In particular, by means of the droplet transfer method, we encapsulate the enzyme, together with a suitable pH indicator, in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) lipid membrane, where differential diffusion of H and urea is ensured by the different permeability () of membranes to the two species. Here we present preliminary tests for the stability of the enzymatic reaction in the presence of lipids and also the successful encapsulation of the enzyme into lipid vesicles.
Miele, Y.
7dd284aa-6a92-4c48-8841-f63e1dbc1a9e
Bánsági, T.
3984187d-60fd-47f2-b6cb-f312dcedadae
Taylor, A.F.
08028a29-428d-4732-b6b1-f7a93389b386
Stano, P.
86e0fc9f-732a-4b19-802e-1aaed7b786b6
Rossi, F.
47d1b9c0-0b75-4936-afb7-feef5fa076f3
2 April 2016
Miele, Y.
7dd284aa-6a92-4c48-8841-f63e1dbc1a9e
Bánsági, T.
3984187d-60fd-47f2-b6cb-f312dcedadae
Taylor, A.F.
08028a29-428d-4732-b6b1-f7a93389b386
Stano, P.
86e0fc9f-732a-4b19-802e-1aaed7b786b6
Rossi, F.
47d1b9c0-0b75-4936-afb7-feef5fa076f3
Miele, Y., Bánsági, T., Taylor, A.F., Stano, P. and Rossi, F.
(2016)
Engineering enzyme-driven dynamic behaviour in lipid vesicles
(Communications in Computer and Information Science),
Abstract
The urea–urease system is a pH dependent enzymatic reaction that was proposed as a convenient model to study pH oscillations in vitro; here, in order to determine the best conditions for oscillations, a two-variable model is used in which acid and substrate, urea, are supplied at rates and from an external medium to an enzyme-containing compartment. Oscillations were observed between pH 4 and 8. Thus the reaction appears a good candidate for the observation of oscillations in experiments, providing the necessary condition that is met. In order to match these conditions, we devised an experimental system where we can ensure the fast transport of acid to the encapsulated urease, compared to that of urea. In particular, by means of the droplet transfer method, we encapsulate the enzyme, together with a suitable pH indicator, in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) lipid membrane, where differential diffusion of H and urea is ensured by the different permeability () of membranes to the two species. Here we present preliminary tests for the stability of the enzymatic reaction in the presence of lipids and also the successful encapsulation of the enzyme into lipid vesicles.
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Published date: 2 April 2016
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Local EPrints ID: 499628
URI: http://eprints.soton.ac.uk/id/eprint/499628
PURE UUID: 6a969efe-fa93-45e2-b7d2-b8b5a1be89fe
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Date deposited: 27 Mar 2025 18:23
Last modified: 28 Mar 2025 03:15
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Author:
Y. Miele
Author:
T. Bánsági
Author:
A.F. Taylor
Author:
P. Stano
Author:
F. Rossi
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