READ ME File for 'Thesis data: Investigating the impact of alterations in a-synuclein on mitochondrial health, mitochondrial quality control and neuronal differentiation'

Dataset DOI: 10.5258/SOTON/D3470

ReadMe Author: NAOMI THORNE, University of Southampton, ORCID: 0000-0001-5364-0253

This dataset supports the thesis entitled "Investigating the impact of alterations in a-synuclein on mitochondrial health, mitochondrial quality control and neuronal differentiation". 
AWARDED BY: University of Southampton
DATE OF AWARD: 2025

DESCRIPTION OF THE DATA

This dataset contains 3 zip files labelled as "Chapter 3", "Chapter 4" and "Chapter 5". Each of these contains zip files for each figure of the thesis.

Chapter 3: Investigating the role of endogenous a-synuclein in mitochondrial health and quality control
Figure 33 - Western blots showing GAPDH and a-synuclein
Figure 34 - Western blots showing B-actin and a-synuclein, excel file showing quantitation of protein 
Figure 35 - Western blots showing B-actin and pS129 a-synuclein, excel file showing quantitation of protein 
Figure 36 - Western blots showing B-actin and a-synuclein, excel file showing quantitation of protein
Figure 37 - Western blots showing B-actin and a-synuclein, excel file showing quantitation of protein
Figure 38 - Western blots showing a-synuclein, a-tubulin, calnexin, CoxII and vinculin 
Figure 39 - Western blots showing GAPDH and a-synuclein
Figure 40 - Fluorescence images showing a-synuclein and DAPI
Figure 41 - Fluorescence images showing a-synuclein and DAPI
Figure 42 & 43 - Excel files showing raw data from ATP assays, Western blots showing a-synuclein, B-actin, CoxII and GAPDH, excel file showing quantitation of protein 
Figure 44 & 45 - Excel files showing raw data from Seahorse MitoStress tests
Figure 46 - Fluorescence images showing TMRM and brightfield, excel file showing quantitation of TMRM fluorescence intensity
Figure 47 - Fluorescence images showing TOM20 and DAPI
Figure 48 & 49 - Excel files showing quantitation of TOM20 morphology
Figure 50 - Western blots showing a-synuclein, B-actin, CoxII, Mfn2 and PINK1, excel files showing quantitation of protein
Figure 51 - Western blots showing a-synuclein, GAPDH and Parkin, excel file showing quantitation of protein
Figure 52 - Fluorescence images showing pUb S65, CytC and DAPI
Figure 53 - Excel files showing quantitation of colocalisation between pUb S65 and CytC
Figure 54 - Western blots showing a-synuclein, GAPDH and LC3, excel file showing quantitation of protein
Figure 55 - Western blots showing a-synuclein, B-actin and LC3, excel file showing quantitation of protein
Figure 56 - Fluorescence images showing LAMP1, CytC and DAPI
Figure 57 & 58 - Excel files showing quantitation of LAMP1 morphology and colocalisation between LAMP1 and CytC
Figure 59 - Fluorescence images showing PDH, TOM20 and DAPI
Figure 60 - Excel file showing quantitation of MDVs


Chapter 4: Investigating the impact of pathological a-synuclein overexpression on mitochondrial health and quality control
Figure 62 - Western blots showing a-synuclein, B-actin and GFP
Figure 63 - Fluorescence images showing a-synuclein, GFP and DAPI
Figure 64 - Western blots showing a-synuclein, GAPDH and GFP 
Figure 65 - Fluorescence images showing a-synuclein, GFP and DAPI
Figure 66 & 67 - Fluorescence images sowing a-synuclein, GFP and DAPI, excel file showing quantitation of GFP puncta 
Figure 68 - Western blots showing pS129 a-synuclein, GAPDH and GFP
Figure 69 - Fluorescence images showing pS129 a-synuclein and DAPI
Figure 70 - Fluorescence images showing a-synuclein, GFP and DAPI
Figure 71 - Western blots showing pS129 a-synuclein, GAPDH and GFP, GraphPad Prism file showing quantitation of protein
Figure 72 - Fluorescence images showing pS129 a-synuclein, TOM20 and DAPI
Figure 73 - Fluorescence images showing pS129 a-synuclein, TOM20 and DAPI
Figure 74 - Western blots showing a-synuclein, a-tubulin, calnexin, CoxII, GFP and vinculin
Figure 75 & 76 - Excel files showing raw data from ATP assays, Western blots showing GFP, B-actin, CoxII and GAPDH, excel file showing quantitation of protein 
Figure 77 & 78 - Excel files showing raw data from Seahorse MitoStress tests
Figure 79 - Fluorescence images showing TMRM and GFP, excel file showing quantitation of TMRM fluorescence intensity 
Figure 80 - Fluorescence images showing GFP, TOM20 and DAPI
Figure 81, 82, 83 & 84 - GraphPad Prism files showing quantitation of TOM20 morphology
Figure 85 & 86 - Western blots showing CoxII, GAPDH and GFP, Western blots showing B-actin, GFP, Mfn2 and PINK1, excel files showing quantitation of protein
Figure 87 - Western blots showing GAPDH, GFP and Parkin, excel file showing quantitation of protein
Figure 88 - Fluorescence images showing CytC, GFP, pUb S65 and DAPI
Figure 89 - Excel file showing quantitation of colocalisation between pUb S65 and CytC
Figure 90 - Western blots showing GFP, GAPDH and LC3, GraphPad Prism file showing quantitation of protein
Figure 91 - Fluorescence images showing CytC, GFP, LAMP1 and DAPI
Figure 92 & 93 - Excel files showing quantitation of LAMP1 morphology and colocalisation between LAMP1 and CytC
Figure 94 - Fluorescence images showing GFP, PDH, TOM20 and DAPI
Figure 95 - Excel file showing quantitation of MDVs


Chapter 5: Investigating the impact of alterations in a-synuclein on neuronal differentiation in SH-SY5Y cells
Figure 97 - Brightfield image panel 
Figure 98 - Fluorescence images showing B-III-tubulin, F-actin and DAPI
Figure 99 - Fluorescence images showing B-III-tubulin and DAPI
Figure 100 - Excel file showing quantitation of B-III-tubulin-positive cells
Figure 101 - Western blots showing B-actin, B-III-tubulin, GAP43 and TH, excel file showing quantitation of protein 
Figure 102 - Fluorescence images showing B-III-tubulin and DAPI
Figure 103 & 104 - Excel files showing quantitation of B-III-tubulin-positive cells
Figure 105 - Western blots showing a-synuclein, B-actin, B-III-tubulin, GAP43 and TH, excel file showing quantitation of protein
Figure 106 - Fluorescence images showing B-III-tubulin and DAPI
Figure 107 & 108 - Excel files showing quantitation of B-III-tubulin-positive cells 
Figure 109 - Fluorescence images showing B-III-tubulin, GFP and DAPI
Figure 110 - Western blots showing a-synuclein, B-actin, B-III-tubulin, GAP43 and TH, excel files showing quantitation of protein
Figure 111 - Fluorescence images showing B-III-tubulin, CytC and DAPI
Figure 112 - Brightfield images 



Date of data collection: 2020-2024

Licence: CCBY

Related projects/Funders: Related to Research Output: Doctoral Thesis 'Investigating the impact of alterations in a-synuclein on mitochondrial health, mitochondrial quality control and neuronal differentiation', Funders: Gerald Kerkut Charitable Trust

Date that file was created: April, 2025