Comparative analysis of protein expression between oesophageal adenocarcinoma and normal adjacent tissue
Comparative analysis of protein expression between oesophageal adenocarcinoma and normal adjacent tissue
Oesophageal adenocarcinoma (OAC) is the 7th most common cancer in the United Kingdom (UK) and remains a significant health challenge. This study presents a proteomic analysis of seven OAC donors complementing our previous neoantigen identification study of their human leukocyte antigen (HLA) immunopeptidomes. Our small UK cohort were selected from donors undergoing treatment for OAC. We used label-free mass spectrometry proteomics to compare OAC tumour tissue to matched normal adjacent tissue (NAT) to quantify expression of 3552 proteins. We identified differential expression of a number of proteins previously linked to OAC and other cancers including common markers of tumourigenesis and immunohistological markers, as well as enrichment of processes and pathways relating to RNA processing and the immune system. Our findings also offer insight into the role of the protein stability in the generation of an OAC neoantigen we previously identified. These results provide independent corroboration of existing oesophageal adenocarcinoma biomarker studies that may inform future diagnostic and therapeutic research.
Adenocarcinoma/metabolism, Aged, Biomarkers, Tumor/metabolism, Esophageal Neoplasms/metabolism, Female, Gene Expression Regulation, Neoplastic, Humans, Male, Middle Aged, Proteomics/methods
Nicholas, Ben
01cab7d7-32c0-413f-804b-d4133070268b
Bailey, Alistair
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McCann, Katy J.
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Walker, Robert C.
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Johnson, Peter
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Elliott, Tim
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Underwood, Tim J.
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Skipp, Paul
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12 March 2025
Nicholas, Ben
01cab7d7-32c0-413f-804b-d4133070268b
Bailey, Alistair
19a50642-79ab-4760-b367-fafd3fccdf01
McCann, Katy J.
154f6b6d-c8b2-43b2-a8a9-ffe243da40c6
Walker, Robert C.
c8fbfe1c-349d-497f-b24e-0295c84c4634
Johnson, Peter
3f6068ce-171e-4c2c-aca9-dc9b6a37413f
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Underwood, Tim J.
8e81bf60-edd2-4b0e-8324-3068c95ea1c6
Skipp, Paul
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
Nicholas, Ben, Bailey, Alistair, McCann, Katy J., Walker, Robert C., Johnson, Peter, Elliott, Tim, Underwood, Tim J. and Skipp, Paul
(2025)
Comparative analysis of protein expression between oesophageal adenocarcinoma and normal adjacent tissue.
PLoS ONE, 20 (3), [e0318572].
(doi:10.1371/journal.pone.0318572).
Abstract
Oesophageal adenocarcinoma (OAC) is the 7th most common cancer in the United Kingdom (UK) and remains a significant health challenge. This study presents a proteomic analysis of seven OAC donors complementing our previous neoantigen identification study of their human leukocyte antigen (HLA) immunopeptidomes. Our small UK cohort were selected from donors undergoing treatment for OAC. We used label-free mass spectrometry proteomics to compare OAC tumour tissue to matched normal adjacent tissue (NAT) to quantify expression of 3552 proteins. We identified differential expression of a number of proteins previously linked to OAC and other cancers including common markers of tumourigenesis and immunohistological markers, as well as enrichment of processes and pathways relating to RNA processing and the immune system. Our findings also offer insight into the role of the protein stability in the generation of an OAC neoantigen we previously identified. These results provide independent corroboration of existing oesophageal adenocarcinoma biomarker studies that may inform future diagnostic and therapeutic research.
Text
journal.pone.0318572
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More information
Accepted/In Press date: 19 January 2025
Published date: 12 March 2025
Keywords:
Adenocarcinoma/metabolism, Aged, Biomarkers, Tumor/metabolism, Esophageal Neoplasms/metabolism, Female, Gene Expression Regulation, Neoplastic, Humans, Male, Middle Aged, Proteomics/methods
Identifiers
Local EPrints ID: 500648
URI: http://eprints.soton.ac.uk/id/eprint/500648
ISSN: 1932-6203
PURE UUID: 70253e3c-01ca-4abf-8e93-ccda04a04dc3
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Date deposited: 07 May 2025 16:57
Last modified: 22 Aug 2025 01:51
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Contributors
Author:
Ben Nicholas
Author:
Alistair Bailey
Author:
Katy J. McCann
Author:
Robert C. Walker
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