Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques
Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques
The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes.
microfluidics, chinese hamster ovary (CHO) cells, confocal microspectroscopy, surface-enhanced raman scattering (SERS), dynamic monitoring
833-840
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Cooper, Jon M.
2dbda23a-e372-4d05-baf1-48b243ced44f
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
February 2008
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Cooper, Jon M.
2dbda23a-e372-4d05-baf1-48b243ced44f
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
Zhang, Xunli, Yin, Huabing, Cooper, Jon M. and Haswell, Stephen J.
(2008)
Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques.
Analytical and Bioanalytical Chemistry, 390 (3), .
(doi:10.1007/s00216-007-1564-9).
(PMID:17849101)
Abstract
The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes.
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e-pub ahead of print date: 12 September 2007
Published date: February 2008
Keywords:
microfluidics, chinese hamster ovary (CHO) cells, confocal microspectroscopy, surface-enhanced raman scattering (SERS), dynamic monitoring
Identifiers
Local EPrints ID: 50092
URI: http://eprints.soton.ac.uk/id/eprint/50092
ISSN: 1618-2642
PURE UUID: dc0746e1-be2d-4e9e-8fa2-5f7fdea0d52c
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Date deposited: 22 Jan 2008
Last modified: 16 Mar 2024 03:55
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Contributors
Author:
Huabing Yin
Author:
Jon M. Cooper
Author:
Stephen J. Haswell
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