READ ME File For "Differentially expressed genes comparing oesophageal adenocarcinoma cell lines treated with an EZH2 inhibitor vs DMSO control"

Dataset DOI: 10.5258/SOTON/D3484

ReadMe Author: OLIVER PICKERING, University of Southampton https://orcid.org/0000-0003-4100-385X

This dataset supports the thesis entitled "The Role of Histone Modifying Enzymes in Oesophageal Adenocarcinoma: Targeting of Enhancer of zeste homolog 2 (EZH2)"
AWARDED BY: University of Southampton
DATE OF AWARD: 2025

DESCRIPTION OF THE DATA
List of differentially expressed genes between OAC cell lines FLO1, MFD-1 and OE33 treated with 5uM tazemetostat, 5uM CPI-1205 or DMSO control for 6 days. 

Novogene Sequencing (Cambridge, UK) for paired-end 150 bp mRNA sequencing at a sequencing depth of 20 million reads per sample using the Illumina Novaseq platform. Analysis of RNA-seq data was performed by Dr Jaya Thomas, Senior Research Fellow in Bioinformatics in the Bioinformatics Research Facility, University of Southampton.


This dataset contains:
- Excel files containing DEG lists for FLO1, MFD-1 and OE33 cells. 


Date of data collection: Dec 2023


Date that the file was created: Feb, 2024