Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
Holmes, Susannah
195f0adc-0b17-43ad-973c-c2d63bb6c2ab
Kirkwood, Henry J.
14eb5309-55fa-4ec1-87fa-ffc18d99b9d1
Bean, Richard
53420357-ca63-48a8-980e-cf8e5259a2dd
Fanghor, Hans
9b7cfab9-d5dc-45dc-947c-2eba5c81a160
11 August 2022
Holmes, Susannah
195f0adc-0b17-43ad-973c-c2d63bb6c2ab
Kirkwood, Henry J.
14eb5309-55fa-4ec1-87fa-ffc18d99b9d1
Bean, Richard
53420357-ca63-48a8-980e-cf8e5259a2dd
Fanghor, Hans
9b7cfab9-d5dc-45dc-947c-2eba5c81a160
Holmes, Susannah, Kirkwood, Henry J. and Bean, Richard
,
et al.
(2022)
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.
Nature Communications, 13, [4708].
(doi:10.1038/s41467-022-32434-6).
Abstract
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
Text
s41467-022-32434-6 (1)
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Accepted/In Press date: 28 July 2022
Published date: 11 August 2022
Identifiers
Local EPrints ID: 501122
URI: http://eprints.soton.ac.uk/id/eprint/501122
ISSN: 2041-1723
PURE UUID: 3e01a3ff-87bc-46e8-8b8f-3de5737a1c69
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Date deposited: 23 May 2025 18:51
Last modified: 22 Aug 2025 01:45
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Author:
Susannah Holmes
Author:
Henry J. Kirkwood
Author:
Richard Bean
Corporate Author: et al.
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