READ ME File For 'OSSS Research Data'

Dataset DOI: 10.5258/SOTON/D3481

Date that the file was created: APRIL, 2025

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GENERAL INFORMATION
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ReadMe Author: SCOTT DAVID ADAMS, University of Southampton [orcid.org/0000-0001-6775-856X]

Date of data collection: NOVEMBER 2020 - NOVEMBER 2024

Information about geographic location of data collection: UNIVERSITY OF SOUTHAMPTON, CENTRE FOR PROTEOMIC RESEARCH

Related projects:
Mechanistic understanding of the relationships between oxidative and electrophilic stress in allergic skin sensitisation [113024489]

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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse: Creative Commons Attribution CC-BY

Recommended citation for the data:

This dataset supports the publication:
AUTHORS: Adams, Scott David
TITLE: Mechanistic understanding of the relationship between oxidative and electrophilic stress in allergic skin sensitisation
JOURNAL:
PAPER DOI IF KNOWN:

Links to other publicly accessible locations of the data:

Links/relationships to ancillary or related data sets: 


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DATA & FILE OVERVIEW
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This dataset contains:

- [OSSS Antioxidant Enzyme Assays (folder, contains readme, 2 subfolders, xlsx file of data extracted from OSSS whole proteomics)]


Relationship between files, if important for context:  each folder has its own readme contained therein

Additional related data collected that was not included in the current data package:

If data was derived from another source, list source:

If there are there multiple versions of the dataset, list the file updated, when and why update was made:



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METHODOLOGICAL INFORMATION
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Description of methods used for collection/generation of data: refer to OSSS thesis chapter 2 (specific sections in subfolder readme files)

Methods for processing the data: refer to OSSS thesis chapter 2 (specific sections in subfolder readme files)

Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: Microsoft Office, R version 4.3 onwards, ImageJ 

Standards and calibration information, if appropriate: refer to OSSS thesis chapter 2 (specific sections in subfolder readme files)

Environmental/experimental conditions: refer to OSSS thesis chapter 2 (specific sections in subfolder readme files)

Describe any quality-assurance procedures performed on the data:refer to OSSS thesis chapter 2 (specific sections in subfolder readme files)

People involved with sample collection, processing, analysis and/or submission: author only


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DATA-SPECIFIC INFORMATION <Create sections for each datafile or set, as appropriate>
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Number of variables:

Number of cases/rows: 

Variable list, defining any abbreviations, units of measure, codes or symbols used:
	- 
   
Missing data codes:

Specialized formats or other abbreviations used:
	- SDA: author's initials
	- DNCB: 2,4-dinitrochlorobenzene
	- H2O2: hydrogen peroxide
	- HaCaT: human keratinocyte cell line
	- DH: combined DNCB + H2O2 4 hours before cell harvest
	- HD2: H2O2 pre-treatment (2 hours) before DNCB treatment (2 hours pre-harvest)
	- HD4: H2O2 pre-treatment (2 hours) before DNCB treatment (4 hours pre-harvest)
	- DI: dual isotope DNCB (1:1 DNCB:DNCB-D3 mixture)
	- DHE: dihydroethidium
	- 4HNE: 4-hydroxy-2-nonenal
	- ACR: acrolein
	- MDA: malondialdehyde
	- GSH: glutathione
	- GSSG: glutathione disulphide
	- SOD: superoxide dismutase
	- C: control
	- VC: vehicle control