READ ME File For 'Dataset Synthesis and biological evaluation of cyclic peptide PROTACs targeting hypoxia inducible factor -1a'

Dataset DOI: 10.5258/SOTON/D3512

Date that the file was created: June, 2025

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GENERAL INFORMATION
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ReadMe Author: Alexander McDermott, University of Southampton 

Date of data collection: ADD IN COLLECTION DATES

Information about geographic location of data collection: Great Britain


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DATA & FILE OVERVIEW
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This dataset contains:

Luciferase data - contains luciferase assay data for both cell viability and activity assays that is recorded on GraphPad.
MST - contains KDs for compounds that bound to HIF-1a, also recorded on GraphPad.
qPCR - contains gene expression response in cancer cells to treatment with cyclic peptide PROTACs. Data analysed on GraphPad. 
Western blot - contains information about the degradation of HIF-1a in cancer cells. Measured using Empiria Studio and data analysed quantitatively on GraphPad.
NMR characterisation - contains characterisation data of compounds analysed on ACD labs.


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METHODOLOGICAL INFORMATION
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Description of methods used for collection/generation of data: detailed in the thesis SI and within the thesis

Methods for processing the data: Luciferase data is analysed with GraphPad, KDs for compounds analysed with GraphPad, qPCR analysed with GraphPad, Western blot analysed with Empiria Software, NMR analysed on ACD labs.

Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: ACD labs Graph Pad, Microsoft excel, Empiria Software.

People involved with sample collection, processing, analysis and/or submission: Alexander McDermott