Purification and functional inactivation of the fission yeast MCMMCM-BP complex
Purification and functional inactivation of the fission yeast MCMMCM-BP complex
The MCM (mini-chromosome maintenance) complex is the core of the eukaryotic replicative helicase and comprises six proteins, Mcm2-Mcm7. In humans, a variant form of the complex has Mcm2 replaced by the MCM-BP protein. Recent results suggest that a similar complex exists in fission yeast with an essential role in DNA replication and cell cycle progression. Here, we describe the purification and subunit composition of the fission yeast MCM(Mcb1) complex. Using newly generated temperature-sensitive alleles, we show that loss of MCM(Mcb1) function leads to accumulation of DNA damage, checkpoint activation and cell cycle arrest, and provide evidence for a role for MCM(Mcb1) in meiosis.
Checkpoint Kinase 1, Chromatography, Affinity, Chromosomes, Fungal/genetics, DNA Damage, DNA Repair, DNA Replication, DNA, Fungal/biosynthesis, DNA-Binding Proteins/metabolism, Mass Spectrometry, Meiosis, Multiprotein Complexes/analysis, Protein Kinases/metabolism, Protein Subunits/analysis, Schizosaccharomyces/cytology, Schizosaccharomyces pombe Proteins/analysis
3850-3855
Li, Juan-juan
7888cf96-2102-4b8c-b719-5dd71f4c359d
Schnick, Jasmin
118bc907-0e8c-43f7-9d1d-b7be92329fd4
Hayles, Jacqueline
015771d6-16d2-458d-a431-07c3b6916c2f
MacNeill, Stuart A
cd09a75f-b26a-4f15-b899-ce451055ebad
15 December 2011
Li, Juan-juan
7888cf96-2102-4b8c-b719-5dd71f4c359d
Schnick, Jasmin
118bc907-0e8c-43f7-9d1d-b7be92329fd4
Hayles, Jacqueline
015771d6-16d2-458d-a431-07c3b6916c2f
MacNeill, Stuart A
cd09a75f-b26a-4f15-b899-ce451055ebad
Li, Juan-juan, Schnick, Jasmin, Hayles, Jacqueline and MacNeill, Stuart A
(2011)
Purification and functional inactivation of the fission yeast MCMMCM-BP complex.
FEBS Letters, 585 (24), .
(doi:10.1016/j.febslet.2011.10.033).
Abstract
The MCM (mini-chromosome maintenance) complex is the core of the eukaryotic replicative helicase and comprises six proteins, Mcm2-Mcm7. In humans, a variant form of the complex has Mcm2 replaced by the MCM-BP protein. Recent results suggest that a similar complex exists in fission yeast with an essential role in DNA replication and cell cycle progression. Here, we describe the purification and subunit composition of the fission yeast MCM(Mcb1) complex. Using newly generated temperature-sensitive alleles, we show that loss of MCM(Mcb1) function leads to accumulation of DNA damage, checkpoint activation and cell cycle arrest, and provide evidence for a role for MCM(Mcb1) in meiosis.
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Accepted/In Press date: 14 October 2011
e-pub ahead of print date: 25 October 2011
Published date: 15 December 2011
Keywords:
Checkpoint Kinase 1, Chromatography, Affinity, Chromosomes, Fungal/genetics, DNA Damage, DNA Repair, DNA Replication, DNA, Fungal/biosynthesis, DNA-Binding Proteins/metabolism, Mass Spectrometry, Meiosis, Multiprotein Complexes/analysis, Protein Kinases/metabolism, Protein Subunits/analysis, Schizosaccharomyces/cytology, Schizosaccharomyces pombe Proteins/analysis
Identifiers
Local EPrints ID: 502112
URI: http://eprints.soton.ac.uk/id/eprint/502112
ISSN: 0014-5793
PURE UUID: f940a45f-74a4-4052-a14d-d4c0e02c70b0
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Date deposited: 17 Jun 2025 16:30
Last modified: 18 Jun 2025 01:56
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Contributors
Author:
Jasmin Schnick
Author:
Jacqueline Hayles
Author:
Stuart A MacNeill
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