READ ME File For Raw Oscilloscope Data for the paper 'Oligonucleotide Selective Detection by Levitated Optomechanics'

Dataset DOI: 10.5258/SOTON/D3576

Date that the file was created: July, 2025

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GENERAL INFORMATION
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ReadMe Author: Timothy Wilson, University of Southampton, ORCID ID: 0009-0003-3289-5881

Date of data collection: Nov 2024 - Jan 2025

Information about geographic location of data collection: B46, University of Southampton, Highfield Campus, UK

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SHARING/ACCESS INFORMATION
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Due to the size (4.4GB), this dataset is available on request from https://library.soton.ac.uk/datarequest

License: CC BY

Recommended citation for the data: 
Timothy Wilson (2025) 'Raw Oscilloscope Data for the paper 'Oligonucleotide Selective Detection by Levitated Optomechanics'', 
University of Southampton, https://doi.org/10.5258/SOTON/D3576

This dataset supports the publication:
AUTHORS: Timothy Wilson, Owen Rackham and Hendrik Ulbricht
TITLE: Oligonucleotide Selective Detection by Levitated Optomechanics


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DATA & FILE OVERVIEW
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Seven folders in a .zip file named as: Standard SiNP, 25A 1000uM ZnCl2 SiNP, 25T 100uM ZnCl2 SiNP, 25T 500uM ZnCl2 SiNP, 25T 750uM ZnCl2 SiNP, 25T 1000uM ZnCl2 SiNP and Compare Peaks. Each dataset is a save of voltage against time in a .bin format. Each set of data has a .bin file containing metadata of oscilloscope data and a .Wfm.bin file which is the waveform save. The files are in pairs in the format Particle_Type_X.bin and Particle_Type_X.Wfm.bin. The Compare Peaks folder are the selected waveforms to visualise PSD comparison in Figure 2.

Figure 2:

This figures compares selected waveforms of the different particle types from the Compare Peaks folder.

(a) Compares Fig_2a_Standard_SiNP to Fig_2a_25A_1000_uM_ZnCl2_SiNP and Fig_2a_25T_1000_uM_ZnCl2_SiNP.
(b) Compares Fig_2b_25T_100_uM_ZnCl2_SiNP to Fig_2b_25T_500_uM_ZnCl2_SiNP, Fig_2b_25T_750_uM_ZnCl2_SiNP and Fig_2b_25T_1000_uM_ZnCl2_SiNP.

Figure 3:

This figure compares groups of data by Linear Discriminant Analysis by Lorentzian curve fitting to the PSDs.

(a) Plots of data in the folders: Standard SiNP, 25A 1000uM ZnCl2 SiNP and 25T 1000uM ZnCl2 SiNP.
(b) Plots of data in the folders: 25T 100uM ZnCl2 SiNP, 25T 500uM ZnCl2 SiNP, 25T 750uM ZnCl2 SiNP and 25T 1000uM ZnCl2 SiNP.

Figure 4:

This figure takes the output of Lorentzian curve fitting to groups of data and does random forest accuracy analysis over 300 MCCV iterations.

(a) Analyses data from the following folders as the orange group: Standard SiNP, 25A 1000uM ZnCl2 SiNP and 25T 1000uM ZnCl2 SiNP.
(b) Analyses data from the following folders as the blue group: 25T 100uM ZnCl2 SiNP, 25T 500uM ZnCl2 SiNP, 25T 750uM ZnCl2 SiNP and 25T 1000uM ZnCl2 SiNP.
(c) Compares feature importances between (a) and (b).

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METHODOLOGICAL INFORMATION
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Silica nanoparticles with and without surface modifications are optically trapped one at a time.
Each dataset is a save of the voltage against time trace caused by the centre-of-mass motion of the trapped particle by detecting the Rayleigh back-scattered light from the trapping field with a single photodetector. 

Methods for processing the data:

Data is obtained as a save file of voltage and time and is processed to a power spectral density.

Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers:

The Python package RTxReadBin (https://www.rohde-schwarz.com/uk/applications/working-with-acquired-waveform-data-in-python-application-card_56279-1165008.html) from Rohde & Schawrz was used to handle the binary files. The Python package Optoanalysis (https://github.com/AshleySetter/optoanalysis) was used for Lorentzian curve fitting.

Environmental/experimental conditions:

This experiment was carried out in a vacuum chamber at 3.5 mbar at room temperature.


People involved with sample collection, processing, analysis and/or submission:

Timothy Wilson.












