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Association between fractional exhaled nitric oxide (FeNO) and cognitive function in patients with obstructive sleep apnea

Association between fractional exhaled nitric oxide (FeNO) and cognitive function in patients with obstructive sleep apnea
Association between fractional exhaled nitric oxide (FeNO) and cognitive function in patients with obstructive sleep apnea
Purpose: Obstructive sleep apnea (OSA) is characterised by intermittent hypoxia and sleep fragmentation, both of which can impair cognition. This study aimed to investigate the association between fractional exhaled nitric oxide (FeNO), a non-invasive marker of airway inflammation, and memory performance in patients with OSA.

Methods: A total of 102 participants were enrolled: 62 with moderate or severe OSA (apnea-hypopnea index, AHI≥15) and 40 with snoring or mild OSA (AHI <15). Memory was assessed with the Rey-Osterrieth Complex Figure Test (RCFT), Digit Ordering Test
(DOT), and Logical Memory Test (LMT). FeNO was measured at 50mL/s (FeNO50) and 200mL/s (FeNO200); alveolar NO (CaNO)
was calculated. Group comparisions used t-tests and chi-square tests, cognitive scores employed mixed-design ANOVA, and
associations were examined with Spearman correlation plus hierarchical regression.

Results: Compared with the snoring or mild OSA group, participants with moderate or severe OSA had larger neck circumference, higher body-mass index, greater daytime sleepiness, and elevated FeNO50 and FeNO200 (P < 0.05). They also showed poorer immediate and delayed visual memory (both P< 0.05), which correlated negatively with AHI (r = −0.088/-0.103, P < 0.05) and FeNO50 (r = −0.286/-0.302, P < 0.05). RCFT scores fell over time (F = 271.171, P < 0.05), with a significant group × time interaction (F = 3.065, P < 0.05). FeNO50 independently predicted poorer immediate recall (β = −0.28, P = 0.018), whereas FeNO200 was not significant.

Conclusion: Moderate or severe OSA is associated with impaired immediate and delayed visual memory. Higher FeNO50 correlates
with memory decline, supporting a link between airway inflammation and cognitive dysfunction in OSA.

Plain language summary: OSA is a common sleep disorder in which breathing repeatedly stops and restarts during the night. This
disrupted sleep can lead to persistent tiredness, poor concentration, and memory problems. In our study, we explored whether a quick, non-invasive breath test called FeNO could flag early memory decline in people with OSA. We found that individuals with more severe OSA had higher FeNO readings and poorer memory scores. These findings suggest that FeNO reflects airway inflammation that may also affect the brain. Our study provides early evidence that this simple breath test could help clinicians identify memory issues in OSA patients sooner and more effectively.
cognitive function, fractional exhaled nitric oxide, obstructive sleep apnea, polysomnography
1179-1608
1603-1614
Zhu, Qilin
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Huang, Lili
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Zhu, Licheng
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Zhang, Xiaobai
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Ji, Honghua
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Niu, Donghua
34731f63-49f2-46aa-9ad8-8d3e352e9fed
Ji, Wangfei
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Ma, Qingqing
afd60aea-b5fe-41ce-8ff2-e30b7f7254db
Chen, Rong
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Shi, Haiyan
df961177-62dc-48bc-b44a-37154ee7b653
Wang, Yihua
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Xu, Lina
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Zhu, Qilin
f9c7f3b1-863f-457e-9c7c-e1177fbe648c
Huang, Lili
97b92602-3817-47f8-a793-1283ab5e1556
Zhu, Licheng
ee798dd3-47c3-4a09-a73a-9ea6acf62ea3
Zhang, Xiaobai
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Ji, Honghua
df06fd9e-28aa-45c0-9cba-91ff55ea3d93
Niu, Donghua
34731f63-49f2-46aa-9ad8-8d3e352e9fed
Ji, Wangfei
06ef2a3d-f805-4291-88d4-6cc7eabdc4e1
Ma, Qingqing
afd60aea-b5fe-41ce-8ff2-e30b7f7254db
Chen, Rong
a1ac0978-8a70-4a5c-978b-efd119ee9c91
Shi, Haiyan
df961177-62dc-48bc-b44a-37154ee7b653
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Xu, Lina
53e9790f-92b8-4fed-95c4-009b878ce0db

Zhu, Qilin, Huang, Lili, Zhu, Licheng, Zhang, Xiaobai, Ji, Honghua, Niu, Donghua, Ji, Wangfei, Ma, Qingqing, Chen, Rong, Shi, Haiyan, Wang, Yihua and Xu, Lina (2025) Association between fractional exhaled nitric oxide (FeNO) and cognitive function in patients with obstructive sleep apnea. Nature and Science of Sleep, 17, 1603-1614. (doi:10.2147/NSS.S524831).

Record type: Article

Abstract

Purpose: Obstructive sleep apnea (OSA) is characterised by intermittent hypoxia and sleep fragmentation, both of which can impair cognition. This study aimed to investigate the association between fractional exhaled nitric oxide (FeNO), a non-invasive marker of airway inflammation, and memory performance in patients with OSA.

Methods: A total of 102 participants were enrolled: 62 with moderate or severe OSA (apnea-hypopnea index, AHI≥15) and 40 with snoring or mild OSA (AHI <15). Memory was assessed with the Rey-Osterrieth Complex Figure Test (RCFT), Digit Ordering Test
(DOT), and Logical Memory Test (LMT). FeNO was measured at 50mL/s (FeNO50) and 200mL/s (FeNO200); alveolar NO (CaNO)
was calculated. Group comparisions used t-tests and chi-square tests, cognitive scores employed mixed-design ANOVA, and
associations were examined with Spearman correlation plus hierarchical regression.

Results: Compared with the snoring or mild OSA group, participants with moderate or severe OSA had larger neck circumference, higher body-mass index, greater daytime sleepiness, and elevated FeNO50 and FeNO200 (P < 0.05). They also showed poorer immediate and delayed visual memory (both P< 0.05), which correlated negatively with AHI (r = −0.088/-0.103, P < 0.05) and FeNO50 (r = −0.286/-0.302, P < 0.05). RCFT scores fell over time (F = 271.171, P < 0.05), with a significant group × time interaction (F = 3.065, P < 0.05). FeNO50 independently predicted poorer immediate recall (β = −0.28, P = 0.018), whereas FeNO200 was not significant.

Conclusion: Moderate or severe OSA is associated with impaired immediate and delayed visual memory. Higher FeNO50 correlates
with memory decline, supporting a link between airway inflammation and cognitive dysfunction in OSA.

Plain language summary: OSA is a common sleep disorder in which breathing repeatedly stops and restarts during the night. This
disrupted sleep can lead to persistent tiredness, poor concentration, and memory problems. In our study, we explored whether a quick, non-invasive breath test called FeNO could flag early memory decline in people with OSA. We found that individuals with more severe OSA had higher FeNO readings and poorer memory scores. These findings suggest that FeNO reflects airway inflammation that may also affect the brain. Our study provides early evidence that this simple breath test could help clinicians identify memory issues in OSA patients sooner and more effectively.

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Accepted/In Press date: 23 June 2025
Published date: 12 July 2025
Keywords: cognitive function, fractional exhaled nitric oxide, obstructive sleep apnea, polysomnography

Identifiers

Local EPrints ID: 503061
URI: http://eprints.soton.ac.uk/id/eprint/503061
ISSN: 1179-1608
PURE UUID: 58784568-97cf-44dd-a394-670b436b441f
ORCID for Yihua Wang: ORCID iD orcid.org/0000-0001-5561-0648

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Date deposited: 18 Jul 2025 17:02
Last modified: 22 Aug 2025 02:13

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Contributors

Author: Qilin Zhu
Author: Lili Huang
Author: Licheng Zhu
Author: Xiaobai Zhang
Author: Honghua Ji
Author: Donghua Niu
Author: Wangfei Ji
Author: Qingqing Ma
Author: Rong Chen
Author: Haiyan Shi
Author: Yihua Wang ORCID iD
Author: Lina Xu

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