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The glucocorticoid receptor gene exon 1-F promoter is not methylated at the NGFI-A binding site in human hippocampus

The glucocorticoid receptor gene exon 1-F promoter is not methylated at the NGFI-A binding site in human hippocampus
The glucocorticoid receptor gene exon 1-F promoter is not methylated at the NGFI-A binding site in human hippocampus
Recent research has demonstrated that early life experience, such as variation in maternal care, can have a profound impact on the physiological and endocrine stress response of Rattus norvegicus. Low maternal care resulted in increased methylation of the nerve growth factor-inducible protein A (NGFI-A, EGR1) binding site located in the hippocampal glucocorticoid receptor gene (Nr3c1) exon 17 promoter, leading to decreased Nr3c1 expression, which results in a reduced efficiency of glucocorticoid-mediated negative feedback on hypothalamus-pituitary-adrenal axis activity. The human glucocorticoid receptor gene (NR3C1) has a highly similar 5’ structure compared to the rat, and the human alternative exon 1-F is the orthologue to the rat exon 17. Based upon the evidence from rats, and the high sequence identity of the regulatory sequences, we examined the methylation pattern of the corresponding NGFI-A binding site in the human glucocorticoid receptor exon 1-F specific promoter in post-mortem hippocampal tissue. In contrast to the findings in rats, neither of the two CpG motifs within the NGFI-A binding site was methylated in the 32 subjects investigated. These observations might reflect different promoter methylation patterns in humans and rats.
behaviour, brain development, cortisol, genetics, personality, stress
1562-2975
262-268
Moser, D.
ca1f99d7-ffb6-413b-ab5c-672d22c5565a
Molitor, A.
e54f3c55-570a-454b-a9eb-ec7013705087
Kumsta, R.
40cfd543-15ea-44fc-b778-3bfc4ae14f6f
Tatschner, T.
b8b20b5b-e118-4aae-a087-6b692e73ce11
Riederer, P.
b7ed9aab-b33e-40ba-afde-ea4a2cb6cc8e
Meyer, J.
50b8fd43-2b12-481c-bb05-9e4bfc38f44a
Moser, D.
ca1f99d7-ffb6-413b-ab5c-672d22c5565a
Molitor, A.
e54f3c55-570a-454b-a9eb-ec7013705087
Kumsta, R.
40cfd543-15ea-44fc-b778-3bfc4ae14f6f
Tatschner, T.
b8b20b5b-e118-4aae-a087-6b692e73ce11
Riederer, P.
b7ed9aab-b33e-40ba-afde-ea4a2cb6cc8e
Meyer, J.
50b8fd43-2b12-481c-bb05-9e4bfc38f44a

Moser, D., Molitor, A., Kumsta, R., Tatschner, T., Riederer, P. and Meyer, J. (2007) The glucocorticoid receptor gene exon 1-F promoter is not methylated at the NGFI-A binding site in human hippocampus. The World Journal of Biological Psychiatry, 8 (4), 262-268. (doi:10.1080/15622970701429862).

Record type: Article

Abstract

Recent research has demonstrated that early life experience, such as variation in maternal care, can have a profound impact on the physiological and endocrine stress response of Rattus norvegicus. Low maternal care resulted in increased methylation of the nerve growth factor-inducible protein A (NGFI-A, EGR1) binding site located in the hippocampal glucocorticoid receptor gene (Nr3c1) exon 17 promoter, leading to decreased Nr3c1 expression, which results in a reduced efficiency of glucocorticoid-mediated negative feedback on hypothalamus-pituitary-adrenal axis activity. The human glucocorticoid receptor gene (NR3C1) has a highly similar 5’ structure compared to the rat, and the human alternative exon 1-F is the orthologue to the rat exon 17. Based upon the evidence from rats, and the high sequence identity of the regulatory sequences, we examined the methylation pattern of the corresponding NGFI-A binding site in the human glucocorticoid receptor exon 1-F specific promoter in post-mortem hippocampal tissue. In contrast to the findings in rats, neither of the two CpG motifs within the NGFI-A binding site was methylated in the 32 subjects investigated. These observations might reflect different promoter methylation patterns in humans and rats.

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More information

Submitted date: March 2007
Published date: 20 July 2007
Keywords: behaviour, brain development, cortisol, genetics, personality, stress

Identifiers

Local EPrints ID: 50307
URI: http://eprints.soton.ac.uk/id/eprint/50307
ISSN: 1562-2975
PURE UUID: d091061c-1ee7-4486-a2e7-735e6a9b9f32

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Date deposited: 14 Feb 2008
Last modified: 15 Mar 2024 10:05

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Contributors

Author: D. Moser
Author: A. Molitor
Author: R. Kumsta
Author: T. Tatschner
Author: P. Riederer
Author: J. Meyer

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