Isolation, differentiation, and characterization of human bone marrow stromal stem cells In Vitro and In Vivo
Isolation, differentiation, and characterization of human bone marrow stromal stem cells In Vitro and In Vivo
In this chapter, we describe techniques for the isolation, enrichment, and characterization of skeletal stem cells from human bone marrow. The methods for enriching STRO-1 and STRO-4 positive cells using magnetic activated cell sorting are detailed, as are the techniques for establishing and characterising osteogenic, adipogenic, and chondrogenic cultures derived from these cells. Finally, we present methods for studying the ability of these enriched skeletal stem and progenitor populations to produce bone in vivo using diffusion chambers following subcutaneous implantation, over 4-6 weeks.
Adipogenesis, Animals, Bone Marrow Cells/cytology, Cell Culture Techniques/methods, Cell Differentiation, Cell Separation/methods, Cells, Cultured, Chondrogenesis, Flow Cytometry, Humans, Mesenchymal Stem Cells/cytology, Mice, Osteogenesis, Adipocyte, Chondrocyte, Diffusion chamber, Stem cells, Bone marrow, Osteoblast
67-85
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Tare, Rahul S
587c9db4-e409-4e7c-a02a-677547ab724a
Tribe, Howard C
1433f1d5-6e04-4d1a-8229-35b9bba9de5e
Stumpf, Patrick
dfdb286c-b321-46d3-8ba2-85b3b4a7f092
Rawlings, Andrew
f7d578ff-a012-4f84-b91f-097736b1f483
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Oreffo, Richard O C
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
1 June 2025
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Tare, Rahul S
587c9db4-e409-4e7c-a02a-677547ab724a
Tribe, Howard C
1433f1d5-6e04-4d1a-8229-35b9bba9de5e
Stumpf, Patrick
dfdb286c-b321-46d3-8ba2-85b3b4a7f092
Rawlings, Andrew
f7d578ff-a012-4f84-b91f-097736b1f483
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Oreffo, Richard O C
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kanczler, Janos, Tare, Rahul S, Tribe, Howard C, Stumpf, Patrick, Rawlings, Andrew, Marshall, Karen and Oreffo, Richard O C
(2025)
Isolation, differentiation, and characterization of human bone marrow stromal stem cells In Vitro and In Vivo.
Methods in molecular biology (Clifton, N.J.), 2885, .
(doi:10.1007/978-1-0716-4306-8_4).
Abstract
In this chapter, we describe techniques for the isolation, enrichment, and characterization of skeletal stem cells from human bone marrow. The methods for enriching STRO-1 and STRO-4 positive cells using magnetic activated cell sorting are detailed, as are the techniques for establishing and characterising osteogenic, adipogenic, and chondrogenic cultures derived from these cells. Finally, we present methods for studying the ability of these enriched skeletal stem and progenitor populations to produce bone in vivo using diffusion chambers following subcutaneous implantation, over 4-6 weeks.
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e-pub ahead of print date: 1 June 2025
Published date: 1 June 2025
Additional Information:
© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Keywords:
Adipogenesis, Animals, Bone Marrow Cells/cytology, Cell Culture Techniques/methods, Cell Differentiation, Cell Separation/methods, Cells, Cultured, Chondrogenesis, Flow Cytometry, Humans, Mesenchymal Stem Cells/cytology, Mice, Osteogenesis, Adipocyte, Chondrocyte, Diffusion chamber, Stem cells, Bone marrow, Osteoblast
Identifiers
Local EPrints ID: 503649
URI: http://eprints.soton.ac.uk/id/eprint/503649
ISSN: 1064-3745
PURE UUID: 87b1b399-0e99-47af-bcf5-a6cd8dc63ed5
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Date deposited: 07 Aug 2025 16:53
Last modified: 01 Oct 2025 02:02
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