Transforming growth factor-beta gene expression studies in nasal mucosal biopsies in naturally occurring allergic rhinitis
Transforming growth factor-beta gene expression studies in nasal mucosal biopsies in naturally occurring allergic rhinitis
INTRODUCTION: Evidence has been provided of enhanced epithelial transforming growth factor-beta (TGF-beta) immunoreactivity in allergic rhinitis, including correlation with intra-epithelial mast cell numbers, and the co-localisation of TGF-beta receptors to mast cells, suggesting that the epithelial expression of TGF-beta may represent an important biological process involved in either the recruitment or retention of mast cells within the epithelium in naturally occurring allergic rhinitis.
PATIENTS AND METHODS: In order to extend the above findings, evaluation was undertaken in whole nasal biopsies from subjects with naturally occurring allergic rhinitis, of levels of TGF-beta isotypes and receptors gene expression using real-time quantitative polymerase chain reaction (TaqMan RT-PCR), and the results compared to those for tumour necrosis factor-alpha (TNF-alpha), as a positive control. The study was also extended to evaluate gene expression for connective tissue growth factor (CTGF) and Smad proteins, as downstream markers of TGF-beta bioactivity, in the same populations.
RESULTS: There were no significant differences between the rhinitic and non-rhinitic groups in the expression of TGF-beta isoforms or Smad-3, Smad-6 and Smad-7 proteins; however, there was increased gene expression for TGF-betaRI and TGF-betaRII along with CTGF in seasonal allergic rhinitis. TNF-alpha gene expression was also increased in seasonal allergic rhinitis, consistent with a more acute inflammatory response in this form of rhinitis.
CONCLUSIONS: This study advances our understanding of the role of TGF-beta in the pathogenesis of the inflammatory response in allergic rhinitis.
Biopsy, Connective Tissue Growth Factor, Gene Expression, Genome, Human, Humans, Immediate-Early Proteins/metabolism, Intercellular Signaling Peptides and Proteins/metabolism, Nasal Mucosa/metabolism, RNA/metabolism, Rhinitis, Allergic, Perennial/genetics, Transforming Growth Factor beta/chemistry, Tumor Necrosis Factor-alpha/metabolism
563-573
Salib, Rami J
d6fde1c1-5b5e-43f7-ae1c-42cce6a0c9fc
September 2007
Salib, Rami J
d6fde1c1-5b5e-43f7-ae1c-42cce6a0c9fc
Salib, Rami J
(2007)
Transforming growth factor-beta gene expression studies in nasal mucosal biopsies in naturally occurring allergic rhinitis.
Annals of The Royal College of Surgeons of England, 89 (6), .
(doi:10.1308/003588407X202164).
Abstract
INTRODUCTION: Evidence has been provided of enhanced epithelial transforming growth factor-beta (TGF-beta) immunoreactivity in allergic rhinitis, including correlation with intra-epithelial mast cell numbers, and the co-localisation of TGF-beta receptors to mast cells, suggesting that the epithelial expression of TGF-beta may represent an important biological process involved in either the recruitment or retention of mast cells within the epithelium in naturally occurring allergic rhinitis.
PATIENTS AND METHODS: In order to extend the above findings, evaluation was undertaken in whole nasal biopsies from subjects with naturally occurring allergic rhinitis, of levels of TGF-beta isotypes and receptors gene expression using real-time quantitative polymerase chain reaction (TaqMan RT-PCR), and the results compared to those for tumour necrosis factor-alpha (TNF-alpha), as a positive control. The study was also extended to evaluate gene expression for connective tissue growth factor (CTGF) and Smad proteins, as downstream markers of TGF-beta bioactivity, in the same populations.
RESULTS: There were no significant differences between the rhinitic and non-rhinitic groups in the expression of TGF-beta isoforms or Smad-3, Smad-6 and Smad-7 proteins; however, there was increased gene expression for TGF-betaRI and TGF-betaRII along with CTGF in seasonal allergic rhinitis. TNF-alpha gene expression was also increased in seasonal allergic rhinitis, consistent with a more acute inflammatory response in this form of rhinitis.
CONCLUSIONS: This study advances our understanding of the role of TGF-beta in the pathogenesis of the inflammatory response in allergic rhinitis.
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Published date: September 2007
Keywords:
Biopsy, Connective Tissue Growth Factor, Gene Expression, Genome, Human, Humans, Immediate-Early Proteins/metabolism, Intercellular Signaling Peptides and Proteins/metabolism, Nasal Mucosa/metabolism, RNA/metabolism, Rhinitis, Allergic, Perennial/genetics, Transforming Growth Factor beta/chemistry, Tumor Necrosis Factor-alpha/metabolism
Identifiers
Local EPrints ID: 503740
URI: http://eprints.soton.ac.uk/id/eprint/503740
ISSN: 0035-8843
PURE UUID: 0a8a735d-dfa5-4b49-8a31-8bc29c535747
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Date deposited: 12 Aug 2025 16:46
Last modified: 13 Aug 2025 02:00
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