Assaying low-density-lipoprotein (LDL) uptake into cells
Assaying low-density-lipoprotein (LDL) uptake into cells
Determination of LDL particle uptake into cells is a valuable technique in the field of cholesterol metabolism. This allows assessment of LDL uptake capacity in different adherent and non-adherent cells types, as well as the effect of cellular, genetic, or pharmacological perturbations on this process. Here, we detail a general procedure that describes the production of fluorescently-labeled LDL particles and quantitative and non-quantitative assays for determining cellular LDL uptake.
Fluorescent Dyes/chemistry, Hep G2 Cells, Humans, Lipoproteins, LDL/chemistry, Staining and Labeling
53-63
Loregger, Anke
35d8c1fe-e11e-481e-bd35-e8160c079231
Nelson, Jessica K.
7dc3f0c8-5a67-4467-a332-efd560a0630b
Zelcer, Noam
c5eff664-b107-4a7c-9823-11ccb8149fbc
2017
Loregger, Anke
35d8c1fe-e11e-481e-bd35-e8160c079231
Nelson, Jessica K.
7dc3f0c8-5a67-4467-a332-efd560a0630b
Zelcer, Noam
c5eff664-b107-4a7c-9823-11ccb8149fbc
Loregger, Anke, Nelson, Jessica K. and Zelcer, Noam
(2017)
Assaying low-density-lipoprotein (LDL) uptake into cells.
In,
Gelissen, Ingrid C. and Brown, Andrew J.
(eds.)
Cholesterol Homeostasis.
(Methods in Molecular Biology, 1583)
.
(doi:10.1007/978-1-4939-6875-6_5).
Record type:
Book Section
Abstract
Determination of LDL particle uptake into cells is a valuable technique in the field of cholesterol metabolism. This allows assessment of LDL uptake capacity in different adherent and non-adherent cells types, as well as the effect of cellular, genetic, or pharmacological perturbations on this process. Here, we detail a general procedure that describes the production of fluorescently-labeled LDL particles and quantitative and non-quantitative assays for determining cellular LDL uptake.
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More information
e-pub ahead of print date: 16 February 2017
Published date: 2017
Keywords:
Fluorescent Dyes/chemistry, Hep G2 Cells, Humans, Lipoproteins, LDL/chemistry, Staining and Labeling
Identifiers
Local EPrints ID: 505334
URI: http://eprints.soton.ac.uk/id/eprint/505334
ISSN: 1064-3745
PURE UUID: 15e53bee-bc71-4fa6-9b3a-25bce7686485
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Date deposited: 07 Oct 2025 16:35
Last modified: 08 Oct 2025 02:17
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Contributors
Author:
Anke Loregger
Author:
Jessica K. Nelson
Author:
Noam Zelcer
Editor:
Ingrid C. Gelissen
Editor:
Andrew J. Brown
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