New regulators of mitosis

Adenekan, Fiyinfoluwa Olufemi (2025) New regulators of mitosis. University of Southampton, Doctoral Thesis, 235pp.

Record type: Thesis (Doctoral)

Abstract

Mitosis is essential for accurate chromosome segregation into daughter cells, and defects in this process can lead to aneuploidy and cancer. The M-Cdk complex (Cyclin-Dependent Kinase 1-Cyclin B) is a key regulator of mitosis, phosphorylating substrates to drive mitotic stages such as prophase and anaphase. While several M-Cdk substrates have been characterised, many remain unexplored.

This thesis focuses on KIAA1671 and KIAA1143, identified from a phosphoproteomic screen of MCdk substrates. KIAA1671 showed a distinct mitotic phenotype and was found to influence spindle stability and actin filament organisation during mitosis and interphase. Its role in actin dynamics and spindle assembly, crucial for spindle positioning and chromosome alignment, was investigated. Loss of KIAA1671 causes spindle elongation and delayed mitosis, emphasising its role in mitotic integrity. Phosphorylation of KIAA1671 in prophase and metaphase suggests a regulatory mechanism controlling its spindle interactions.

KIAA1143 localises to RNA-processing foci and plays a role in cell cycle regulation. Its depletion disrupts mitotic timing, implicating it in the control of cell cycle progression.

Text

New Cell Regulators of Mitosis - Version of Record

Restricted to Repository staff only until 27 February 2026.

Available under License University of Southampton Thesis Licence.

Text

Final-thesis-submission-Examination-Mr-Fiyin-Adenekan

Restricted to Repository staff only