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Assessing the gut microbiota composition in older adults: connections to physical activity and healthy ageing

Assessing the gut microbiota composition in older adults: connections to physical activity and healthy ageing
Assessing the gut microbiota composition in older adults: connections to physical activity and healthy ageing
The composition and functionality of the gut microbiota (GM) changes throughout the life course. As we move into older age, it starts to shift towards a less healthy one, which may lead to an imbalance in the GM community. Strategies that can reverse age-related dysbiosis are an important part of healthy aging. Little is known about the GM composition of older adults with different physical activity (PA) levels and whether it might contribute to healthy ageing. The aim of this study was to compare the GM composition of older adults with different PA levels and assess if it is associated with healthy ageing. 101 participants aged between 65–85 years undertook anthropometric measures, a 6-min walking test, wore an accelerometer for 7 days and provided a faecal sample. Faecal GM composition was analysed using 16S rRNA sequencing. We found that those who fulfilled the WHO/UK PA recommendations had higher relative abundance of several health-related bacteria such as Lactobacillus, F. prausnitzii and Roseburia intestinalis and lower abundance of disease-associated bacteria such as D.piger or Enterobacterales when compared to those who did not reach PA recommendations. These findings suggest that PA might improve the GM composition and has the potential to, at least partially, revert age-associated dysbiosis and promote healthy ageing.
Ageing, Elderly, Exercise, Gut microbiome, Gut microbiota, Healthy ageing, Older adults, Physical activity
2509-2723
6039-6063
Ramos, Catarina
a2baeb04-0398-4063-881b-ec495cbcd08c
Magistro, Daniele
ab9296bc-fda6-469e-a3f8-3a574faa1b7e
Walton, Gemma E.
a167f493-b3bf-4063-acdf-acdfa8c1137c
Whitham, Anya
432351bb-a896-4b38-9620-5a8201754143
Camp, Nicola
9dcf59fe-47c4-43db-bb6a-333721427eb9
Poveda, Carlos
ab3bec40-8034-418f-bd8f-7cfbad7b856f
Gibson, Glenn R.
24ac4753-4f78-475f-9766-5da179e2ab92
Hough, John
4d508286-cff3-4104-81d2-eb7b45aa3de6
Kinnear, Will
3268f0ff-39e5-4889-a4e6-414381e6da62
Hunter, Kirsty
91861278-241c-41ad-9aae-189d8939f695
Ramos, Catarina
a2baeb04-0398-4063-881b-ec495cbcd08c
Magistro, Daniele
ab9296bc-fda6-469e-a3f8-3a574faa1b7e
Walton, Gemma E.
a167f493-b3bf-4063-acdf-acdfa8c1137c
Whitham, Anya
432351bb-a896-4b38-9620-5a8201754143
Camp, Nicola
9dcf59fe-47c4-43db-bb6a-333721427eb9
Poveda, Carlos
ab3bec40-8034-418f-bd8f-7cfbad7b856f
Gibson, Glenn R.
24ac4753-4f78-475f-9766-5da179e2ab92
Hough, John
4d508286-cff3-4104-81d2-eb7b45aa3de6
Kinnear, Will
3268f0ff-39e5-4889-a4e6-414381e6da62
Hunter, Kirsty
91861278-241c-41ad-9aae-189d8939f695

Ramos, Catarina, Magistro, Daniele, Walton, Gemma E., Whitham, Anya, Camp, Nicola, Poveda, Carlos, Gibson, Glenn R., Hough, John, Kinnear, Will and Hunter, Kirsty (2025) Assessing the gut microbiota composition in older adults: connections to physical activity and healthy ageing. GeroScience, 47 (4), 6039-6063. (doi:10.1007/s11357-025-01605-w).

Record type: Article

Abstract

The composition and functionality of the gut microbiota (GM) changes throughout the life course. As we move into older age, it starts to shift towards a less healthy one, which may lead to an imbalance in the GM community. Strategies that can reverse age-related dysbiosis are an important part of healthy aging. Little is known about the GM composition of older adults with different physical activity (PA) levels and whether it might contribute to healthy ageing. The aim of this study was to compare the GM composition of older adults with different PA levels and assess if it is associated with healthy ageing. 101 participants aged between 65–85 years undertook anthropometric measures, a 6-min walking test, wore an accelerometer for 7 days and provided a faecal sample. Faecal GM composition was analysed using 16S rRNA sequencing. We found that those who fulfilled the WHO/UK PA recommendations had higher relative abundance of several health-related bacteria such as Lactobacillus, F. prausnitzii and Roseburia intestinalis and lower abundance of disease-associated bacteria such as D.piger or Enterobacterales when compared to those who did not reach PA recommendations. These findings suggest that PA might improve the GM composition and has the potential to, at least partially, revert age-associated dysbiosis and promote healthy ageing.

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s11357-025-01605-w - Version of Record
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More information

Accepted/In Press date: 5 March 2025
Published date: 17 March 2025
Additional Information: Publisher Copyright: © Crown 2025.
Keywords: Ageing, Elderly, Exercise, Gut microbiome, Gut microbiota, Healthy ageing, Older adults, Physical activity

Identifiers

Local EPrints ID: 505945
URI: http://eprints.soton.ac.uk/id/eprint/505945
ISSN: 2509-2723
PURE UUID: eb9b4779-1f1a-4867-ab57-bd761735efd1
ORCID for Daniele Magistro: ORCID iD orcid.org/0000-0002-2554-3701

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Date deposited: 23 Oct 2025 17:06
Last modified: 24 Oct 2025 02:14

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Contributors

Author: Catarina Ramos
Author: Daniele Magistro ORCID iD
Author: Gemma E. Walton
Author: Anya Whitham
Author: Nicola Camp
Author: Carlos Poveda
Author: Glenn R. Gibson
Author: John Hough
Author: Will Kinnear
Author: Kirsty Hunter

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