Contribution of cyclic di-GMP in the control of type III and type VI secretion in pseudomonas aeruginosa
Contribution of cyclic di-GMP in the control of type III and type VI secretion in pseudomonas aeruginosa
Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.
Animals, Bacterial Toxins/metabolism, Cyclic GMP/analogs & derivatives, Moths/microbiology, Mutation, Pseudomonas aeruginosa/genetics, Type III Secretion Systems/genetics, Type VI Secretion Systems/genetics
213-224
McCarthy, Ronan R
0b2cf2e0-b0ff-4c92-aa04-92d91182d1f2
Valentini, Martina
5ed4a18f-fd92-44e7-8c5e-ba4ff489ca73
Filloux, Alain
6f79a42e-17d2-4fb1-bab9-a27c41a5b029
2017
McCarthy, Ronan R
0b2cf2e0-b0ff-4c92-aa04-92d91182d1f2
Valentini, Martina
5ed4a18f-fd92-44e7-8c5e-ba4ff489ca73
Filloux, Alain
6f79a42e-17d2-4fb1-bab9-a27c41a5b029
McCarthy, Ronan R, Valentini, Martina and Filloux, Alain
(2017)
Contribution of cyclic di-GMP in the control of type III and type VI secretion in pseudomonas aeruginosa.
Methods in molecular biology (Clifton, N.J.), 1657, .
(doi:10.1007/978-1-4939-7240-1_17).
Abstract
Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.
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Published date: 2017
Keywords:
Animals, Bacterial Toxins/metabolism, Cyclic GMP/analogs & derivatives, Moths/microbiology, Mutation, Pseudomonas aeruginosa/genetics, Type III Secretion Systems/genetics, Type VI Secretion Systems/genetics
Identifiers
Local EPrints ID: 506095
URI: http://eprints.soton.ac.uk/id/eprint/506095
ISSN: 1064-3745
PURE UUID: 015e891e-83b4-412d-9ce7-f58e1b37bfbf
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Date deposited: 28 Oct 2025 18:25
Last modified: 29 Oct 2025 03:15
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Author:
Ronan R McCarthy
Author:
Martina Valentini
Author:
Alain Filloux
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