Inactivation of African Swine Fever Virus by reagents commonly used in containment laboratories
Inactivation of African Swine Fever Virus by reagents commonly used in containment laboratories
Rapid and effective virus inactivation is an essential step for safe diagnostic testing and for research and vaccine development using infectious viruses. We characterised the reduction of African Swine Fever Virus (ASFV) infectivity using Virkon™ S (Lanxess) 1% w/v disinfectant, FACS™ Lysing buffer (BD), and AVL™ buffer (Qiagen), using porcine cell culture. No virus was detected following a 30 s 20:1 v/v mixing ratio of Virkon™ S 1% with high titre ASFV, supporting its effective use as a laboratory surface disinfectant. FACS™ Lysing and AVL™ buffers also inactivated ASFV, permitting safe removal of treated infected samples from high containment facilities.
McCleary, Stephen
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McCarthy, Ronan R.
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Strong, Rebecca
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Edwards, Jane
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Crooke, Helen
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15 June 2021
McCleary, Stephen
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McCarthy, Ronan R.
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Strong, Rebecca
66abc51c-3f2a-44b1-bcaa-21477bfd67e9
Edwards, Jane
4f3ac03d-6393-4459-a2c3-55153be4f8b1
Crooke, Helen
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McCleary, Stephen, McCarthy, Ronan R., Strong, Rebecca, Edwards, Jane and Crooke, Helen
(2021)
Inactivation of African Swine Fever Virus by reagents commonly used in containment laboratories.
Journal of Virological Methods, 295, [114203].
(doi:10.1016/j.jviromet.2021.114203).
Abstract
Rapid and effective virus inactivation is an essential step for safe diagnostic testing and for research and vaccine development using infectious viruses. We characterised the reduction of African Swine Fever Virus (ASFV) infectivity using Virkon™ S (Lanxess) 1% w/v disinfectant, FACS™ Lysing buffer (BD), and AVL™ buffer (Qiagen), using porcine cell culture. No virus was detected following a 30 s 20:1 v/v mixing ratio of Virkon™ S 1% with high titre ASFV, supporting its effective use as a laboratory surface disinfectant. FACS™ Lysing and AVL™ buffers also inactivated ASFV, permitting safe removal of treated infected samples from high containment facilities.
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Published date: 15 June 2021
Identifiers
Local EPrints ID: 506146
URI: http://eprints.soton.ac.uk/id/eprint/506146
ISSN: 0166-0934
PURE UUID: 5f6b087f-b8e1-42bf-96c3-d38fb7051bb5
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Date deposited: 29 Oct 2025 17:36
Last modified: 01 Nov 2025 03:12
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Contributors
Author:
Stephen McCleary
Author:
Ronan R. McCarthy
Author:
Rebecca Strong
Author:
Jane Edwards
Author:
Helen Crooke
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