Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I
Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I
The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing noncoding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within the minor satellite repeats. Depletion of minor satellite transcripts delayed progression through meiosis I by activation of the spindle assembly checkpoint. Arrested oocytes had poorly congressed chromosomes, and centromeres were frequently split by microtubules. Thus, we have demonstrated that the centromeric RNA plays a specific role in female meiosis I compared with mitosis and is required for maintaining the structural integrity of centromeres. This may contribute to the high aneuploidy rates observed in female meiosis.
Wu, Tianyu
e4245a76-e630-475f-94e3-ce37ab25b13a
Lane, Simon I.R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Morgan, Stephanie L.
3f975d1c-0af5-4e8e-871b-359733ff4026
Tang, Feng
02af10b0-8191-4d46-aec1-24b88220f132
Jones, Keith T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
4 October 2021
Wu, Tianyu
e4245a76-e630-475f-94e3-ce37ab25b13a
Lane, Simon I.R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Morgan, Stephanie L.
3f975d1c-0af5-4e8e-871b-359733ff4026
Tang, Feng
02af10b0-8191-4d46-aec1-24b88220f132
Jones, Keith T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
Wu, Tianyu, Lane, Simon I.R., Morgan, Stephanie L., Tang, Feng and Jones, Keith T.
(2021)
Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.
Journal of Cell Biology, 220 (10).
(doi:10.1083/jcb.202011153).
Abstract
The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing noncoding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within the minor satellite repeats. Depletion of minor satellite transcripts delayed progression through meiosis I by activation of the spindle assembly checkpoint. Arrested oocytes had poorly congressed chromosomes, and centromeres were frequently split by microtubules. Thus, we have demonstrated that the centromeric RNA plays a specific role in female meiosis I compared with mitosis and is required for maintaining the structural integrity of centromeres. This may contribute to the high aneuploidy rates observed in female meiosis.
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Published date: 4 October 2021
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Local EPrints ID: 506583
URI: http://eprints.soton.ac.uk/id/eprint/506583
ISSN: 1540-8140
PURE UUID: 474807f2-22d8-4e70-9332-8f04b0515ec3
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Date deposited: 11 Nov 2025 17:57
Last modified: 12 Nov 2025 02:44
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Author:
Tianyu Wu
Author:
Simon I.R. Lane
Author:
Stephanie L. Morgan
Author:
Feng Tang
Author:
Keith T. Jones
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