READ ME File For Dataset for "Breakdown of microbial networks links nutrient stress and reef coral disease".

Dataset DOI: 10.5258/SOTON/D3751

Date that the file was created: November, 2025

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GENERAL INFORMATION
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ReadMe Author: Raphaela Gracie, University of Southampton [OPTIONAL add ORCID ID]

Date of data collection: 2022

Information about geographic location of data collection: The Coral Reef Laboratory


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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse: CC-BY

Recommended citation for the data:

This dataset supports the publication:
AUTHORS: Raphaela Gracie, Jörg Wiedenmann, Phyllis Lam, Michael Sweet and Cecilia D’Angelo
TITLE: Breakdown of microbial networks links nutrient stress and reef coral disease
JOURNAL: 
PAPER DOI IF KNOWN:

Links to other publicly accessible locations of the data:

Links/relationships to ancillary or related data sets: 


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DATA & FILE OVERVIEW
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This dataset contains:

The metadata for the corals included in this study are contained within:
metadata.csv

Physiological data for Fv/Fm and tissue loss are in:
fvfm.csv
tissue_loss.csv

The 16S rRNA sequencing data are contained within the following files:
taxonomy_table.csv
counts_table.csv


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METHODOLOGICAL INFORMATION
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Description of methods used for collection/generation of data: Samples for sequencing were collected using sterile scalpel and flash frozen in liquid nitrogen before DNA extraction and Illumina sequencing.

Methods for processing the data: Taxonomic classification was assigned to ASVs using DECIPHER and the SILVA SSU r138 (version 2, 2024) database.

Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: Metadata and physiological data files may be viewed in Excel. The sequencing data can be processed using R and RStudio.

Environmental/experimental conditions: These samples were collected from corals cultured in the experimental aquaria of The Coral Reef Laboratory. Further details can be found at: https://doi.org/10.1017/S0025315411001883.


People involved with sample collection, processing, analysis and/or submission: Raphaela Gracie