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Determination of nitrate and phosphate in seawater at nanomolar concentrations

Determination of nitrate and phosphate in seawater at nanomolar concentrations
Determination of nitrate and phosphate in seawater at nanomolar concentrations
Over much of the world’s surface oceans, nitrate and phosphate concentrations are below the limit of detection (LOD) of conventional techniques of analysis. However, these nutrients play a controlling role in primary productivity and carbon sequestration in these waters. In recent years, techniques have been developed to address this challenge, and methods are now available for the shipboard analysis of nanomolar (nM) nitrate and phosphate concentrations with a high sample throughput.
This article provides an overview of the methods for nM nitrate and phosphate analysis in seawater. We outline in detail a system comprising liquid waveguide capillary cells connected to a conventional segmented-flow autoanalyser and using miniaturised spectrophotometers. This approach is suitable for routine field measurements of nitrate and phosphate and achieves LODs of 0.8 nM phosphate and 1.5 nM nitrate.
Liquid-waveguide-capillary cell, Nanomolar concentration, Nitrate, Nutrient, Ocean, Phosphate, Seawater, Segmented-flow autoanalyser, Spectrophotometer
0165-9936
169-182
Patey, M.D.
953bb9e5-e90f-4178-ae55-114c025137d2
Rijkenberg, M.J.A.
b801d0be-bd6a-4dbf-beb0-48a777fe5b64
Statham, P.J.
51458f15-d6e2-4231-8bba-d0567f9e440c
Mowlem, M.
6f633ca2-298f-48ee-a025-ce52dd62124f
Stinchcombe, M.C.
433dd398-15f7-4730-9f1e-992d65bec70b
Achterberg, E.P.
685ce961-8c45-4503-9f03-50f6561202b9
Patey, M.D.
953bb9e5-e90f-4178-ae55-114c025137d2
Rijkenberg, M.J.A.
b801d0be-bd6a-4dbf-beb0-48a777fe5b64
Statham, P.J.
51458f15-d6e2-4231-8bba-d0567f9e440c
Mowlem, M.
6f633ca2-298f-48ee-a025-ce52dd62124f
Stinchcombe, M.C.
433dd398-15f7-4730-9f1e-992d65bec70b
Achterberg, E.P.
685ce961-8c45-4503-9f03-50f6561202b9

Patey, M.D., Rijkenberg, M.J.A., Statham, P.J., Mowlem, M., Stinchcombe, M.C. and Achterberg, E.P. (2008) Determination of nitrate and phosphate in seawater at nanomolar concentrations. Trends in Analytical Chemistry, 27 (2), 169-182. (doi:10.1016/j.trac.2007.12.006).

Record type: Article

Abstract

Over much of the world’s surface oceans, nitrate and phosphate concentrations are below the limit of detection (LOD) of conventional techniques of analysis. However, these nutrients play a controlling role in primary productivity and carbon sequestration in these waters. In recent years, techniques have been developed to address this challenge, and methods are now available for the shipboard analysis of nanomolar (nM) nitrate and phosphate concentrations with a high sample throughput.
This article provides an overview of the methods for nM nitrate and phosphate analysis in seawater. We outline in detail a system comprising liquid waveguide capillary cells connected to a conventional segmented-flow autoanalyser and using miniaturised spectrophotometers. This approach is suitable for routine field measurements of nitrate and phosphate and achieves LODs of 0.8 nM phosphate and 1.5 nM nitrate.

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More information

Submitted date: 6 December 2007
Published date: February 2008
Keywords: Liquid-waveguide-capillary cell, Nanomolar concentration, Nitrate, Nutrient, Ocean, Phosphate, Seawater, Segmented-flow autoanalyser, Spectrophotometer
Organisations: Ocean and Earth Science, National Oceanography Centre,Southampton

Identifiers

Local EPrints ID: 50740
URI: http://eprints.soton.ac.uk/id/eprint/50740
ISSN: 0165-9936
PURE UUID: f871ed61-f2ee-4e3e-8bae-a8e91152891d
ORCID for M. Mowlem: ORCID iD orcid.org/0000-0001-7613-6121

Catalogue record

Date deposited: 19 Mar 2008
Last modified: 16 Mar 2024 03:08

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Contributors

Author: M.D. Patey
Author: M.J.A. Rijkenberg
Author: P.J. Statham
Author: M. Mowlem ORCID iD
Author: M.C. Stinchcombe
Author: E.P. Achterberg

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