Ronan, McCarthy (Inventors) (2024) Enhancing the activity of a plastic-degrading enzyme. GB2625586.
Abstract
Enhancing the activity of a plastic-degrading enzyme, a biofilm-producing micro-organism is modified to exhibit elevated levels of cyclic di-GMP. The enzyme can be a PETase. The micro organism, such as E. coli, can be modified to express a heterologous diguanylate cyclase such as DdcC or WspR. An expression construct for use in the method encodes an enzyme that modulates levels of cyclic di-GMP, such as diguanylate cyclase, and also a plastic degrading enzyme, such as a PETase. The diguanylate cyclase can be an E. coli diguanylate cyclase or a P. aeruginosa diguanylate cyclase.
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