Identification and antimicrobial susceptibility testing of MRSA using miniaturized fluidic wells laser-patterned on transparent substrate

Ali, Ahsan, T. Peter, Kessiya, T.B, Krishna, Swaminathan T., Raja, Saeed, Kordo, Dushianthan, Ahilanandan, Sivaraman, Gopalan Krishnan and Sones, Collin (2026) Identification and antimicrobial susceptibility testing of MRSA using miniaturized fluidic wells laser-patterned on transparent substrate. In Photonics West. SPIE.. (doi:10.1117/12.3079350).

Abstract

Existing bacterial detection methods unfortunately are limited in their inability to detect and identify pathogens that typically occur at low concentrations. Miniaturized microfluidic devices (or lab-on-a-chip systems) which use small volumes of liquid samples and reagents, are inexpensive to fabricate, easy to use and do not require expensive ancillary instrumentation, if included in point-of-care testing pathways within under-resourced settings, can provide timely guidance towards infectious disease management and thus a unique opportunity to combat the global crisis of antibiotic resistance. This work reports the identification and antibiotic susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA) using well-like structures (with ~mm2 dimensions) designed to miniaturise and mimic the bacteriological testing routinely performed using agar petri-plates. With optimally identified parameters for use with the laser direct-write patterned wells we report the detection of MRSA (ATCC 43300) spiked in fish samples with a limit-of-detection of 1000 CFU/ml and Minimum Inhibitory Concentration of 32μg/ml of Oxacillin.

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Contributors

Author: Ahsan Ali

Author: Kordo Saeed

Author: Ahilanandan Dushianthan

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