Novel fluorescent proteins for high-content screening
Novel fluorescent proteins for high-content screening
The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.
1054-1060
Wolff, M.
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Wiedenmann, J.
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Nienhaus, G.U.
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Valler, M.
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Heilker, R.
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December 2006
Wolff, M.
f4474770-dda8-4ed9-8704-1c28569c4e83
Wiedenmann, J.
ad445af2-680f-4927-90b3-589ac9d538f7
Nienhaus, G.U.
8f2b40ea-be57-4b32-880f-8a0e5c1585aa
Valler, M.
80fea6e5-4e02-4a96-9625-cf89e278c809
Heilker, R.
8d99f47e-fa07-407f-a649-138d1e6ec789
Wolff, M., Wiedenmann, J., Nienhaus, G.U., Valler, M. and Heilker, R.
(2006)
Novel fluorescent proteins for high-content screening.
Drug Discovery Today, 11 (23-24), .
(doi:10.1016/j.drudis.2006.09.005).
Abstract
The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.
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Published date: December 2006
Identifiers
Local EPrints ID: 51168
URI: http://eprints.soton.ac.uk/id/eprint/51168
ISSN: 1359-6446
PURE UUID: acd24cf2-3e9d-44d3-ae2f-882b696a5340
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Date deposited: 08 May 2008
Last modified: 16 Mar 2024 03:53
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Contributors
Author:
M. Wolff
Author:
G.U. Nienhaus
Author:
M. Valler
Author:
R. Heilker
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