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Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor: is cis-trans isomerization involved?

Record type: Article

Proteins from the family of the green fluorescent protein (GFP) are presently extensively used in molecular and cellular biology. Recent studies suggest that isomerization of the chromophore occurs upon excitation and is involved in nonradiative deactivation. Using Raman spectroscopy, we report on photoinduced cis-trans isomerization in the red fluorescent protein eqFP611 from the sea anemone Entacmaea quadricolor. The crystal structure of eqFP611 shows that the chemical structure of the chromophore, p-hydroxybenzylidene-imidazolinone with an extended -conjugated system, is nearly identical to the chromophore of other red fluorescent proteins such as DsRed and HcRed. However, the chromophore of eqFP611 has a trans configuration whereas the chromophore of DsRed has a cis configuration. Upon irradiation with 532-nm light, the absorption of eqFP611 peaking at 559 nm diminished, and concomitantly a drastic decrease in the quantum yield of fluorescence as well as more complex decay kinetics was observed. Upon irradiation, changes in the Raman spectrum of eqFP611 were observed, and the relative intensities and peak positions of the irradiated eqFP611 showed striking similarity with the peaks in the Raman spectrum of DsRed. These observations are tentatively interpreted as trans-to-cis isomerization of the chromophore taking place upon irradiation together with the opening of new, nonradiative pathways.

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Citation

Loos, D.C., Habuchi, S., Flors, C., Hotta, J-I., Wiedenmann, J., Nienhaus, G.U. and Hofkens, J. (2006) Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor: is cis-trans isomerization involved? Journal of the American Chemical Society, 128, (19), pp. 6270-6271. (doi:10.1021/ja0545113 S0002-7863(05)04511-7).

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Published date: 2006

Identifiers

Local EPrints ID: 51180
URI: http://eprints.soton.ac.uk/id/eprint/51180
ISSN: 0002-7863
PURE UUID: 7c03d147-1685-4aaa-9b79-63f841547097
ORCID for J. Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

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Date deposited: 08 May 2008
Last modified: 17 Jul 2017 14:49

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Contributors

Author: D.C. Loos
Author: S. Habuchi
Author: C. Flors
Author: J-I. Hotta
Author: J. Wiedenmann ORCID iD
Author: G.U. Nienhaus
Author: J. Hofkens

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