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Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications

Record type: Article

EosFP is a fluorescent protein from the coral Lobophyllia hemprichii that changes its fluorescence emission from green to red upon irradiation with near-UV light. Here we present the spectroscopic properties of wild-type EosFP and a variety of monomeric and dimeric mutants and provide a structural interpretation of its oligomerization and photoconversion, which is based on X-ray structure analysis of the green and red species that we reported recently. Because functional expression of the monomeric EosFP variant is limited to temperatures of 30°C, we have developed a tandem dimer. This construct, in which two EosFP subunits are connected by a flexible 12 amino acid linker, expresses well after fusion with the androgen and endothelin A receptors at 37°C. A variety of applications in cellular imaging, developmental biology and automated high-content screening applications are presented, which demonstrate that EosFP is a powerful tool for in vivo monitoring of cellular processes.

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Citation

Nienhaus, G. Ulrich, Nienhaus, Karin, Hölzle, Angela, Ivanchenko, Sergey, Renzi, Fabiana, Oswald, Franz, Wolff, Michael, Schmitt, Florian, Röcker, Carlheinz, Vallone, Beatrice, Weidemann, Wolfgang, Heilker, Ralf, Nar, Herbert and Wiedenmann, Jörg (2006) Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications Photochemistry and Photobiology, 82, (2), pp. 351-358. (doi:10.1562/2005-05-19-RA-533).

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Published date: March 2006

Identifiers

Local EPrints ID: 51183
URI: http://eprints.soton.ac.uk/id/eprint/51183
ISSN: 0031-8655
PURE UUID: ea07a486-af32-4d0c-ba73-5215411f9d85
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

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Date deposited: 08 May 2008
Last modified: 17 Jul 2017 14:49

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Contributors

Author: G. Ulrich Nienhaus
Author: Karin Nienhaus
Author: Angela Hölzle
Author: Sergey Ivanchenko
Author: Fabiana Renzi
Author: Franz Oswald
Author: Michael Wolff
Author: Florian Schmitt
Author: Carlheinz Röcker
Author: Beatrice Vallone
Author: Wolfgang Weidemann
Author: Ralf Heilker
Author: Herbert Nar

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