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Cracks in the -can: fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria)

Cracks in the -can: fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria)
Cracks in the -can: fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria)
We characterize two green fluorescent proteins (GFPs), an orange fluorescent protein, and a nonfluorescent red protein isolated from the sea anemone Anemonia sulcata. The orange fluorescent protein and the red protein seem to represent two different states of the same protein. Furthermore, we describe the cloning of a GFP and a nonfluorescent red protein. Both proteins are homologous to the GFP from Aequorea victoria. The red protein is significantly smaller than other GFP homologues, and the formation of a closed GFP-like -can is not possible. Nevertheless, the primary structure of the red protein carries all features necessary for orange fluorescence. We discuss a type of -can that could be formed in a multimerization process.
0027-8424
14091-14096
Wiedenmann, J.
ad445af2-680f-4927-90b3-589ac9d538f7
Elke, C.
da52735a-92f0-4fc8-860c-529455865445
Spindler, K-D.
24aace55-70a9-40a8-9853-d7436e6de644
Funke, W.
4c874d76-e2b8-4829-942d-ef91695cf300
Wiedenmann, J.
ad445af2-680f-4927-90b3-589ac9d538f7
Elke, C.
da52735a-92f0-4fc8-860c-529455865445
Spindler, K-D.
24aace55-70a9-40a8-9853-d7436e6de644
Funke, W.
4c874d76-e2b8-4829-942d-ef91695cf300

Wiedenmann, J., Elke, C., Spindler, K-D. and Funke, W. (2000) Cracks in the -can: fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria). Proceedings of the National Academy of Sciences, 97 (26), 14091-14096.

Record type: Article

Abstract

We characterize two green fluorescent proteins (GFPs), an orange fluorescent protein, and a nonfluorescent red protein isolated from the sea anemone Anemonia sulcata. The orange fluorescent protein and the red protein seem to represent two different states of the same protein. Furthermore, we describe the cloning of a GFP and a nonfluorescent red protein. Both proteins are homologous to the GFP from Aequorea victoria. The red protein is significantly smaller than other GFP homologues, and the formation of a closed GFP-like -can is not possible. Nevertheless, the primary structure of the red protein carries all features necessary for orange fluorescence. We discuss a type of -can that could be formed in a multimerization process.

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More information

Published date: 19 December 2000
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 51185
URI: https://eprints.soton.ac.uk/id/eprint/51185
ISSN: 0027-8424
PURE UUID: c1b13e30-8f30-4008-a8a2-812992260696
ORCID for J. Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 08 May 2008
Last modified: 14 Mar 2019 01:40

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Contributors

Author: J. Wiedenmann ORCID iD
Author: C. Elke
Author: K-D. Spindler
Author: W. Funke

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