The University of Southampton
University of Southampton Institutional Repository

Targeted green-red photoconversion of EosFP, a fluorescent marker protein

Ivanchenko, S., Röcker, C., Oswald, F., Wiedenmann, J. and Nienhaus, G.U. (2005) Targeted green-red photoconversion of EosFP, a fluorescent marker protein Journal of Biological Physics, 31, (3-4), pp. 249-259. (doi:10.1007/s10867-005-0174-z).

Record type: Article


EosFP is a novel fluorescent protein from the stony coral Lobophyllia hemprichii. Its gene was cloned in Escherichia coli to express the tetrameric wild-type protein. The protein emits strong green fluorescence (516 nm) that shifts toward red (581 nm) upon near-ultraviolet irradiation at ?390 nm due to a photo-induced modification that involves a break in the peptide backbone next to the chromophore. Using site-directed mutagenesis, dimeric (d1EosFP, d2EosFP) and monomeric (mEosFP) variants were produced with essentially unaltered spectroscopic properties. Here we present a spectroscopic characterization of EosFP and its variants, including room- and low-temperature spectra, fluorescence lifetime determinations, two-photon excitation and two-photon photoconversion. Furthermore, by transfection of a human cancer (HeLa) cell with a fusion construct of a mitochondrial targeting sequence and d2EosFP, we demonstrate how localized photoconversion of EosFP can be employed for resolving intracellular processes.

Full text not available from this repository.

More information

Published date: December 2005
Keywords: fluorescent protein - fusion protein - two-photon excitation - fluorescence spectroscopy - photoconversion


Local EPrints ID: 51186
ISSN: 0092-0606
PURE UUID: b28bea03-4c3e-4cac-93f0-e20a7e04ad4b
ORCID for J. Wiedenmann: ORCID iD

Catalogue record

Date deposited: 08 May 2008
Last modified: 17 Jul 2017 14:49

Export record



Author: S. Ivanchenko
Author: C. Röcker
Author: F. Oswald
Author: J. Wiedenmann ORCID iD
Author: G.U. Nienhaus

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.