READ ME File For 'Dataset for thesis "The role of endometrial gland-derived extracellular vesicles in mediating an optimal uterine environment"' Dataset DOI: 10.5258/SOTON/PG/T230 ReadMe Author: MOLLY RUTT, University of Southampton 0000-0003-4360-9820 This dataset supports the thesis entitled The role of endometrial gland-derived extracellular vesicles in mediating an optimal uterine environment AWARDED BY: University of Southampton DATE OF AWARD: 2026 Date of data collection: 2021-2026 Information about geographic location of data collection: n/a Licence: CC BY Related projects/Funders: The role of endometrial gland-derived extracellular vesicles in mediating an optimal uterine environment -------------------- DATA & FILE OVERVIEW -------------------- This dataset contains: 5 zip files, one for each chapter of the thesis and one for all protocols Chapter 3 EV characterization: contains 5 folders: chapter 3 flow, chapter 3 microscopy, chapter 3 miRNA qPCR, chapter 3 NTA and chapter 3 RNAseq. Chapter 4 RPL vs controls Chapter 5 organoids Chapter 6 NOEL Protocols: contains all protocols used for data collection in above files Relationship between files, if important for context: N/a Additional related data collected that was not included in the current data package: Patient data securely stored in Princess Anne Hospital Research Office If data was derived from another source, list source: N/a -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: Data was produced using techniques detailed in the included zip file "protocols" Methods for processing the data: Data was processed using FlowJo (for flow cytometry data), Image J (for fluorescence microscopy), Microscopy Image Browser (for SBFSEM), or in excel. Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: Flowjo v10.10.0, Microscopy Image Browser, Image J Standards and calibration information, if appropriate: N/a Environmental/experimental conditions: Biological sample variance, before and after treatment with Lipiodol. Describe any quality-assurance procedures performed on the data: N/a People involved with sample collection, processing, analysis and/or submission: Molly Rutt, Ella Proudley, Jennifer Pearson Farr, Alexandra Kermack, Ying Cheong, Ian Reddin.