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Identification of GFP-like proteins in non-bioluminescent, azooxanthellate Anthozoa opens new perspectives for bioprospecting

Record type: Article

We screened nonbioluminescent, azooxanthellate cnidaria as potential sources for advanced marker proteins and succeeded in cloning a tetrameric green fluorescent protein (GFP) from the tentacles of Cerianthus membranaceus. The fluorescence of this protein (cmFP512) is characterized by excitation maximum at 503 nm, emission maximum at 512 nm, extinction coefficient of 58,800 M–1 cm–1, quantum yield of 0.66, and fluorescence lifetime of 2.4 ns. The chromophore is formed from the tripeptide Gln-Tyr-Gly. The amino acid sequence of this protein shares 17.8% identical residues with GFP from Aequorea victoria. Weak interactions between the subunits of the tetramer make cmFP512 a promising lead structure for the generation of monomeric variants of fluorescent proteins. Both red fluorescent proteins and nonfluorescent proteins of the GFP family were also purified from tissue homogenates of Adamsia palliata and Calliactis parasitica. The results presented here indicate that a photoprotective function of GFP-like proteins is unlikely in the examined anthozoa species

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Citation

Wiedenmann, J., Ivanchenko, S., Oswald, F. and Nienhaus, G.U. (2004) Identification of GFP-like proteins in non-bioluminescent, azooxanthellate Anthozoa opens new perspectives for bioprospecting Marine Biotechnology, 6, (3), pp. 270-277. (doi:10.1007/s10126-004-3006-4).

More information

Published date: June 2004
Keywords: red fluorescent protein, green fluorescent protein, anthozoa, photoprotection

Identifiers

Local EPrints ID: 51211
URI: http://eprints.soton.ac.uk/id/eprint/51211
ISSN: 1436-2228
PURE UUID: ab455470-7b0b-4119-8de5-72eda602fa58
ORCID for J. Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 12 May 2008
Last modified: 17 Jul 2017 14:49

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Contributors

Author: J. Wiedenmann ORCID iD
Author: S. Ivanchenko
Author: F. Oswald
Author: G.U. Nienhaus

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