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Dimeric variants of the red fluorescent protein eqFP611 generated by site-directed mutagenesis

Wiedenmann, J., Vallone, B., Renzi, F., Nienhaus, K., Ivanchenko, S., Röcker, C. and Nienhaus, G.U., (2004) Dimeric variants of the red fluorescent protein eqFP611 generated by site-directed mutagenesis Savitsky, A.P., Brovko, L.Y., Bornhop, D.J., Raghavachari, R. and Achilefu, S.I. (eds.) In Genetically Engineered and Optical Probes for Biomedical Applications II. International Society for Optical Engineering. 296 pp, pp. 23-29. (doi:10.1117/12.529370).

Record type: Conference or Workshop Item (Paper)


The red fluorescent protein eqFP611 shows favorable properties for applications as molecular marker. Its usefulness is, however, limited by its tendency to form tetramers at physiological concentrations. To provide a basis for the rational design of monomeric variants, we examined the monomer interfaces in the x-ray structure of eqFP611. The arrangement of the four ß cans is very similar to that of other GFP-like proteins such as DsRed and RTMS5. In eqFP611, the monomers are linked by comparatively weak interactions, as inferred from the dissociation into monomers in the presence of SDS or at high dilution. Analysis at the single-molecule level revealed that the monomers are highly fluorescent. Some structural features of the tetrameric interfaces explain the weak subunit interactions in eqFP611. Functional dimeric variants could be generated by altering the A/B interface by single point mutations (Thr122Arg, Val124Thr). By contrast, structural manipulations in the A/C interface resulted as yet in essentially complete loss of fluorescence. Presumably, the folding of eqFP611 into its functional form relies on A/C interfacial interactions.

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Published date: 14 June 2004
Additional Information: Actually deposited by Jane Conquer using existing entry as template
Venue - Dates: Genetically Engineered and Optical Probes for Biomedical Applications II, 2004-01-01 - 2004-01-01


Local EPrints ID: 51215
ISBN: 9780819452375
PURE UUID: f85442cb-c585-4164-9d6c-56d46335f27c
ORCID for J. Wiedenmann: ORCID iD

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Date deposited: 12 May 2008
Last modified: 17 Jul 2017 14:49

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Author: J. Wiedenmann ORCID iD
Author: B. Vallone
Author: F. Renzi
Author: K. Nienhaus
Author: S. Ivanchenko
Author: C. Röcker
Author: G.U. Nienhaus
Editor: A.P. Savitsky
Editor: L.Y. Brovko
Editor: D.J. Bornhop
Editor: R. Raghavachari
Editor: S.I. Achilefu

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