Diffractive micro bar codes for encoding of biomolecules in multiplexed assays
Diffractive micro bar codes for encoding of biomolecules in multiplexed assays
Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several superimposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.
1902-1909
Broder, Graham R.
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Ranasinghe, Rohan T.
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She, Joseph K.
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Banu, Shahanara
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Birtwell, Sam W.
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Cavalli, Gabriel
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Galitonov, Gerasim S.
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Holmes, David
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Martins, Hugo F.P.
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MacDonald, Kevin F.
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Neylon, Cameron
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Zheludev, Nikolay
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Roach, Peter L.
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Morgan, Hywel
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15 March 2008
Broder, Graham R.
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Ranasinghe, Rohan T.
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She, Joseph K.
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Banu, Shahanara
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Birtwell, Sam W.
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Cavalli, Gabriel
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Galitonov, Gerasim S.
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Holmes, David
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Martins, Hugo F.P.
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MacDonald, Kevin F.
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Neylon, Cameron
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Zheludev, Nikolay
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Roach, Peter L.
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Morgan, Hywel
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Broder, Graham R., Ranasinghe, Rohan T., She, Joseph K., Banu, Shahanara, Birtwell, Sam W., Cavalli, Gabriel, Galitonov, Gerasim S., Holmes, David, Martins, Hugo F.P., MacDonald, Kevin F., Neylon, Cameron, Zheludev, Nikolay, Roach, Peter L. and Morgan, Hywel
(2008)
Diffractive micro bar codes for encoding of biomolecules in multiplexed assays.
Analytical Chemistry, 80 (6), .
(doi:10.1021/ac7018574).
Abstract
Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several superimposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.
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Published date: 15 March 2008
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Local EPrints ID: 52037
URI: http://eprints.soton.ac.uk/id/eprint/52037
ISSN: 0003-2700
PURE UUID: d953e118-997a-40f6-81f2-ffc9713fb0ed
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Date deposited: 09 Jun 2008
Last modified: 16 Mar 2024 03:36
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Contributors
Author:
Graham R. Broder
Author:
Rohan T. Ranasinghe
Author:
Joseph K. She
Author:
Shahanara Banu
Author:
Sam W. Birtwell
Author:
Gabriel Cavalli
Author:
Gerasim S. Galitonov
Author:
David Holmes
Author:
Hugo F.P. Martins
Author:
Kevin F. MacDonald
Author:
Cameron Neylon
Author:
Nikolay Zheludev
Author:
Peter L. Roach
Author:
Hywel Morgan
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