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Diffractive micro bar codes for encoding of biomolecules in multiplexed assays

Diffractive micro bar codes for encoding of biomolecules in multiplexed assays
Diffractive micro bar codes for encoding of biomolecules in multiplexed assays
Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several superimposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.
0003-2700
1902-1909
Broder, Graham R.
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Ranasinghe, Rohan T.
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She, Joseph K.
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Banu, Shahanara
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Birtwell, Sam W.
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Cavalli, Gabriel
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Galitonov, Gerasim S.
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Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Martins, Hugo F.P.
d3c0a265-fcd4-4dc7-bdff-6b3cb5642dd6
MacDonald, Kevin F.
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Neylon, Cameron
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Zheludev, Nikolay
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Roach, Peter L.
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Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Broder, Graham R.
6e8722f8-020d-4df6-85a1-30f2f5fdf419
Ranasinghe, Rohan T.
b29fc8b4-2a66-430a-85fa-ff1c9c261f32
She, Joseph K.
6624f7c6-aa57-4663-a06c-d77700fba7c4
Banu, Shahanara
5e1caab9-665e-4a17-b582-28a059acdf06
Birtwell, Sam W.
40be7fb5-3cc9-4a4a-8e8f-97e13a0d9b40
Cavalli, Gabriel
37d26614-5388-4c24-b5ad-531715d1ddb7
Galitonov, Gerasim S.
94d18a31-b65a-4d0f-b7c8-45468afcb24e
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Martins, Hugo F.P.
d3c0a265-fcd4-4dc7-bdff-6b3cb5642dd6
MacDonald, Kevin F.
76c84116-aad1-4973-b917-7ca63935dba5
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
Zheludev, Nikolay
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Roach, Peter L.
ca94060c-4443-482b-af3e-979243488ba9
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Broder, Graham R., Ranasinghe, Rohan T., She, Joseph K., Banu, Shahanara, Birtwell, Sam W., Cavalli, Gabriel, Galitonov, Gerasim S., Holmes, David, Martins, Hugo F.P., MacDonald, Kevin F., Neylon, Cameron, Zheludev, Nikolay, Roach, Peter L. and Morgan, Hywel (2008) Diffractive micro bar codes for encoding of biomolecules in multiplexed assays. Analytical Chemistry, 80 (6), 1902-1909. (doi:10.1021/ac7018574).

Record type: Article

Abstract

Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several superimposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.

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Published date: 15 March 2008

Identifiers

Local EPrints ID: 52037
URI: https://eprints.soton.ac.uk/id/eprint/52037
ISSN: 0003-2700
PURE UUID: d953e118-997a-40f6-81f2-ffc9713fb0ed
ORCID for Kevin F. MacDonald: ORCID iD orcid.org/0000-0002-3877-2976
ORCID for Nikolay Zheludev: ORCID iD orcid.org/0000-0002-1013-6636
ORCID for Peter L. Roach: ORCID iD orcid.org/0000-0001-9880-2877
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 09 Jun 2008
Last modified: 14 Mar 2019 01:53

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Contributors

Author: Graham R. Broder
Author: Rohan T. Ranasinghe
Author: Joseph K. She
Author: Shahanara Banu
Author: Sam W. Birtwell
Author: Gabriel Cavalli
Author: Gerasim S. Galitonov
Author: David Holmes
Author: Hugo F.P. Martins
Author: Cameron Neylon
Author: Peter L. Roach ORCID iD
Author: Hywel Morgan ORCID iD

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