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Diffractive micro bar codes for encoding of biomolecules in multiplexed assays.

Broder, Graham R., Ranasinghe, Rohan T., She, Joseph K., Banu, Shahanara, Birtwell, Sam W., Cavalli, Gabriel, Galitonov, Gerasim S., Holmes, David, Martins, Hugo F.P., MacDonald, Kevin F., Neylon, Cameron, Zheludev, Nikolay, Roach, Peter L. and Morgan, Hywel (2008) Diffractive micro bar codes for encoding of biomolecules in multiplexed assays. Analytical Chemistry, 80, (6), pp. 1902-1909. (doi:10.1021/ac7018574).

Record type: Article


Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several superimposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.

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Published date: 15 March 2008


Local EPrints ID: 52037
ISSN: 0003-2700
PURE UUID: d953e118-997a-40f6-81f2-ffc9713fb0ed
ORCID for Kevin F. MacDonald: ORCID iD
ORCID for Hywel Morgan: ORCID iD

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Date deposited: 09 Jun 2008
Last modified: 17 Jul 2017 14:46

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Author: Graham R. Broder
Author: Rohan T. Ranasinghe
Author: Joseph K. She
Author: Shahanara Banu
Author: Sam W. Birtwell
Author: Gabriel Cavalli
Author: Gerasim S. Galitonov
Author: David Holmes
Author: Hugo F.P. Martins
Author: Cameron Neylon
Author: Peter L. Roach
Author: Hywel Morgan ORCID iD

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