Diffractive micro bar codes for encoding of biomolecules in multiplexed assays.


Broder, Graham R., Ranasinghe, Rohan T., She, Joseph K., Banu, Shahanara, Birtwell, Sam W., Cavalli, Gabriel, Galitonov, Gerasim S., Holmes, David, Martins, Hugo F.P., MacDonald, Kevin F., Neylon, Cameron, Zheludev, Nikolay, Roach, Peter L. and Morgan, Hywel (2008) Diffractive micro bar codes for encoding of biomolecules in multiplexed assays. Analytical Chemistry, 80, (6), pp. 1902-1909. (doi:10.1021/ac7018574).

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Description/Abstract

Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several superimposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1021/ac7018574
ISSNs: 0003-2700 (print)
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ePrint ID: 52037
Date :
Date Event
15 March 2008Published
Date Deposited: 09 Jun 2008
Last Modified: 16 Apr 2017 18:01
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/52037

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